2. Academic Validation
  3. Novel bioactive molecules from Lentzea violacea strain AS 08 using one strain-many compounds (OSMAC) approach

Novel bioactive molecules from Lentzea violacea strain AS 08 using one strain-many compounds (OSMAC) approach

  • Bioorg Med Chem Lett. 2017 Jun 1;27(11):2579-2582. doi: 10.1016/j.bmcl.2017.03.075.
Aehtesham Hussain 1,  Muzafar A Rather 2,  Mohd S Dar 3,  Mushtaq A Aga 4,  Nisar Ahmad 4,  Aabid Manzoor 5,  Arem Qayum 3,  Aiyatullah Shah 5,  Saleem Mushtaq 5,  Zahoor Ahmad 2,  Qazi P Hassan 6
Affiliations

Affiliations

  • 1 Microbial Biotechnology Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar, Srinagar, Jammu & Kashmir 190005, India; Academy of Scientific and Innovative Research, CSIR - Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi, 180001, India.
  • 2 Clinical Microbiology and PK/PD Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar, Srinagar, Jammu & Kashmir 190005, India.
  • 3 Cancer Pharmacology Division, CSIR - Indian Institute of Integrative Medicine, Jammu Tawi, 180001, India.
  • 4 Medicinal Chemistry Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar, Srinagar, Jammu & Kashmir 190005, India.
  • 5 Microbial Biotechnology Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar, Srinagar, Jammu & Kashmir 190005, India.
  • 6 Microbial Biotechnology Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar, Srinagar, Jammu & Kashmir 190005, India; Academy of Scientific and Innovative Research, CSIR - Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi, 180001, India. Electronic address: qphassan@iiim.ac.in.
PMID: 28400238 DOI: 10.1016/j.bmcl.2017.03.075
Abstract

A new eudesmane sesquiterpenoid (1), and a new homologue of virginiae butanolide E (2) along with butyl isobutyl phthalate (3) were isolated from, actinomycete-Lentzea violacea strain AS08 isolated from north western Himalayas by stressing on modified one strain-many compounds (OSMAC) method. The structures of the new compounds were elucidated by extensive spectroscopic analyses including 1D, 2D NMR along with HR-ESI-MS and FT-IR data. Herein, a distinctive method was added for inspecting secretory profile of the strain by quantification of extract value of cell free supernatant in different types of culture media fallowed by HPLC profiling of respective extracts, which revealed a highly altered metabolic profile of the strain and formed the base for the selection of media. The compounds 1 and 2 showed moderate activity against Gram negative (MIC ∼32-64µgml-1) in comparison to Gram positive Bacterial pathogens. Compound 1 exhibited significant activity in human cancerous cell lines (IC50 ∼19.2µM).

Keywords

Actinomycetes; Antitumor and antimicrobial activity; Bioactive compounds; Lentzea violacea.

Figures