2. Academic Validation
  3. Chromodomain protein CDYL is required for transmission/restoration of repressive histone marks

Chromodomain protein CDYL is required for transmission/restoration of repressive histone marks

  • J Mol Cell Biol. 2017 Jun 1;9(3):178-194. doi: 10.1093/jmcb/mjx013.
Yongqing Liu 1,  Shumeng Liu 1,  Shuai Yuan 1,  Huajing Yu 1,  Yu Zhang 1,  Xiaohan Yang 1,  Guojia Xie 1,  Zhe Chen 1,  Wanjin Li 1,  Bosen Xu 1,  Luyang Sun 1,  Yongfeng Shang 1 2 3,  Jing Liang 1
Affiliations

Affiliations

  • 1 Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
  • 2 Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.
  • 3 Tianjin Key Laboratory of Medical Epigenetics, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.
PMID: 28402439 DOI: 10.1093/jmcb/mjx013
Abstract

Faithful transmission or restoration of epigenetic information such as repressive histone modifications through generations is critical for the maintenance of cell identity. We report here that chromodomain Y-like protein (CDYL), a chromodomain-containing transcription corepressor, is physically associated with chromatin assembly factor 1 (CAF-1) and the replicative helicase MCM complex. We showed that CDYL bridges CAF-1 and MCM, facilitating histone transfer and deposition during DNA replication. We demonstrated that CDYL recruits histone-modifying Enzymes G9a, SETDB1, and EZH2 to replication forks, leading to the addition of H3K9me2/3 and H3K27me2/3 on newly deposited histone H3. Significantly, depletion of CDYL impedes early S phase progression and sensitizes cells to DNA damage. Our data indicate that CDYL plays an important role in the transmission/restoration of repressive histone marks, thereby preserving the epigenetic landscape for the maintenance of cell identity.

Keywords

CAF-1; CDYL; MCM; epigenetic inheritance; histone modification.

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