2. Academic Validation
  3. Hirsutane-Type Sesquiterpenes with Inhibitory Activity of Microglial Nitric Oxide Production from the Red Alga-Derived Fungus Chondrostereum sp. NTOU4196

Hirsutane-Type Sesquiterpenes with Inhibitory Activity of Microglial Nitric Oxide Production from the Red Alga-Derived Fungus Chondrostereum sp. NTOU4196

  • J Nat Prod. 2017 May 26;80(5):1615-1622. doi: 10.1021/acs.jnatprod.7b00196.
George Hsiao 1,  Wei-Chiung Chi 2 3,  Ka-Lai Pang 3,  Jih-Jung Chen 4,  Yueh-Hsiung Kuo 5,  Yu-Kai Wang 6,  Hyo-Jung Cha 3,  Shen-Chieh Chou,  Tzong-Huei Lee 6
Affiliations

Affiliations

  • 1 Graduate Institute of Medical Science and Department of Pharmacology, College of Medicine, Taipei Medical University , Taipei 11031, Taiwan.
  • 2 Department of Food Science, National Quemoy University , Kinmen 89250, Taiwan.
  • 3 Institute of Marine Biology, National Taiwan Ocean University , Keelung 20224, Taiwan.
  • 4 School of Pharmaceutical Sciences, National Yang-Ming University , Taipei 11221, Taiwan.
  • 5 Department of Biotechnology, Asia University , Taichung 41354, Taiwan.
  • 6 Institute of Fisheries Science, National Taiwan University , Taipei 10617, Taiwan.
PMID: 28514148 DOI: 10.1021/acs.jnatprod.7b00196
Abstract

The marine red alga Pterocladiella capillacea is an economic alga for the food industry in Taiwan, and its associated highly diversified fungi have not been investigated meticulously thus far. The EtOAc extract of the fermented broth of Chondrostereum sp. NTOU4196, a Fungal strain isolated from P. capillacea, was found to exhibit significant nitric oxide (NO) production inhibitory activity in lipopolysaccharide-activated murine RAW 264.7 cells at a concentration of 100 μg/mL in the preliminary screening. Therefore, separation of the active principles from the fermented broths was performed, and that has led to the isolation of eight new 5,5,5-tricyclic hirsutane-type Sesquiterpenes, namely, chondroterpenes A-H (1-8), together with seven known analogues. They were identified by analyses of spectroscopic data and comparison with literature values. Among the new isolates, chondroterpene A (1) exhibited more significant NO production inhibitory activity in murine BV-2 microglial cells, and of all the isolated compounds, hirsutanol A (9) exerted limited cytotoxic effects and the most potent inhibitory activity on NO production.

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