2. Academic Validation
  3. Transitional metaplasia in intestinal epithelium of rats submitted to intestinal cystoplasty and treatment with L -lysine

Transitional metaplasia in intestinal epithelium of rats submitted to intestinal cystoplasty and treatment with L -lysine

  • Acta Cir Bras. 2017 Apr;32(4):297-306. doi: 10.1590/s0102-865020170040000297.
Alessandra Marques Dos Santos 1,  Joao Paulo Ferreira Coelho 2,  Camila de Carvalho Juanes 2,  Rafael Barbosa de Azevedo 2,  Clara Araujo Diniz 3,  Francisco Vagnaldo Fechine Jamacaru 4,  Conceição Aparecida Dornelas 5
Affiliations

Affiliations

  • 1 Fellow Master degree, Postgraduate Program in Pathology, Department of Pathology, Universidade Federal do Ceará (UFC), Brazil. Intellectual and scientific content of the study, histopathological examinations, technical procedures, manuscript preparation.
  • 2 Graduate student, Scientific Iniciation Program, UFC, Fortaleza-CE, Brazil. Technical procedures.
  • 3 Graduate student, Scientific Iniciation Program, UFC, Fortaleza-CE, Brazil. Acquisition of data.
  • 4 PhD, Researcher, Núcleo de Pesquisa e Desenvolvimento de Medicamentos (NPDM), School of Medicine, UFC, Fortaleza-CE, Brazil. Analysis and interpretation of data, statistical analysis.
  • 5 PhD, Associate Professor, Department of Pathology, UFC, Fortaleza-CE, Brazil. Conception and design of the study, technical procedures, critical revision, supervised all phases of the study.
PMID: 28538804 DOI: 10.1590/s0102-865020170040000297
Abstract

Purpose:: To evaluated the effects of L-lysine on the intestinal and urothelial epithelia in cystoplasty in rats.

Methods:: Twenty-eight 9-week-old rats were assigned to 4 groups: Group A (n=8) cystoplasty followed by administration of L-lysine (150 mg/kg body weight by gavage) for 30 weeks; Group B (n=8) cystoplasty + water for 30 weeks; Group C (n=6) L-lysine for 30 weeks; Group D (n=6) water for 30 weeks.

Results:: On histopathology with hematoxylin and eosin, mild to moderate hyperplasia transitional was observed in at the site of anastomosis in all Animals submitted to cystoplasty (Groups A and B), but "transitional metaplasia" of the intestinal glandular epithelium was more accentuated in Group A (p=0.045). No inflammatory cells, dysplasia or abnormalities were observed. Staining with Alcian blue revealed a substantial reduction of goblet cells and mucins in the colon segment (Groups A and B).

Conclusion:: The administration of L-lysine to rats accelerated the development of transitional metaplasia in the epithelium of the colon segment in cystoplasty.

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