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  2. Human M2-type pyruvate kinase: cDNA cloning, chromosomal assignment and expression in hepatoma

Human M2-type pyruvate kinase: cDNA cloning, chromosomal assignment and expression in hepatoma

  • Gene. 1988 Dec 20;73(2):509-16. doi: 10.1016/0378-1119(88)90515-x.
K Tani 1 M C Yoshida H Satoh K Mitamura T Noguchi T Tanaka H Fujii S Miwa
Affiliations

Affiliation

  • 1 Department of Internal Medicine, University of Tokyo, Japan.
Abstract

Two overlapping clones, covering the entire coding sequence of human M2-type Pyruvate Kinase (PK) cDNA, were isolated and sequenced. Nucleotide Sequencing results showed that they contained the 109-bp 5'-untranslated region, the 1593-bp coding region and the 585-bp 3'-untranslated region. Nucleotide sequence homology was 90% and 69% with rat M2-type and L-type PK cDNA, respectively. In situ hybridization using the human M2-type PK cDNA probe disclosed that the gene for M2-type PK is located at band q22 on chromosome 15. Northern blot analysis with RNA from human hepatoma demonstrated that M2-type PK was predominantly expressed in hepatoma cells, whereas L-type PK was preferentially expressed in the non-tumor portion of the liver.

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