2. Academic Validation
  3. Anti-inflammatory effects and corresponding mechanisms of cirsimaritin extracted from Cirsium japonicum var. maackii Maxim

Anti-inflammatory effects and corresponding mechanisms of cirsimaritin extracted from Cirsium japonicum var. maackii Maxim

  • Bioorg Med Chem Lett. 2017 Jul 15;27(14):3076-3080. doi: 10.1016/j.bmcl.2017.05.051.
Myoung-Sook Shin 1,  Jun Yeon Park 2,  Jaemin Lee 3,  Hye Hyun Yoo 4,  Dae-Hyun Hahm 5,  Sang Cheon Lee 6,  Sanghyun Lee 3,  Gwi Seo Hwang 2,  Kiwon Jung 7,  Ki Sung Kang 8
Affiliations

Affiliations

  • 1 Natural Constituents Research Center, Korea Institute of Science and Technology Gangneung, Institute of Natural Products, 210-340, Republic of Korea.
  • 2 College of Korean Medicine, Gachon University, Seongnam 13120, Republic of Korea.
  • 3 Department of Integrative Plant Science, Chung-Ang University, Anseong 17546, Republic of Korea.
  • 4 Institute of Pharmaceutical Science and Technology and College of Pharmacy, Hanyang University, Ansan 426-791, Republic of Korea.
  • 5 Acupuncture and Meridian Science Research Center, Kyung Hee University, Seoul 02447, Republic of Korea.
  • 6 Imsil Research Institute of Cheese Science, Imsil 566-881, Republic of Korea.
  • 7 Institute of Pharmaceutical Sciences, College of Pharmacy, CHA University, Sungnam 13844, Republic of Korea. Electronic address: pharmj@cha.ac.kr.
  • 8 College of Korean Medicine, Gachon University, Seongnam 13120, Republic of Korea. Electronic address: kkang@gachon.ac.kr.
PMID: 28554870 DOI: 10.1016/j.bmcl.2017.05.051
Abstract

In this study, we investigated the anti-inflammatory effects and mechanisms of cirsimaritin isolated from an ethanol extract of the aerial parts of Cirsium japonicum var. maackii Maxim. using RAW264.7 cells. The extract and its flavonoid cirsimaritin inhibited nitric oxide (NO) production and inducible nitric oxide synthase expression in RAW264.7 cells. Cirsimaritin inhibited interleukin-6, tumor necrosis factor-α, and NO production in a concentration-dependent manner in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. From a western blot study, pretreatment with cirsimaritin inhibited phosphorylation/degradation of IκBα and phosphorylation of Akt in LPS-stimulated RAW264.7 cells. Moreover, cirsimaritin suppressed activation of LPS-induced transcription factors, such as c-Fos and signal transducer and activator of transcription 3 (STAT3), in RAW264.7 cells. Collectively, these results show that cirsimaritin possesses anti-inflammatory activity, which is regulated by inhibition of c-Fos and STAT3 phosphorylation in RAW264.7 cells.

Keywords

Cirsimaritin; Cirsium japonicum; Inflammation.

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