2. Academic Validation
  3. Conifer Diterpene Resin Acids Disrupt Juvenile Hormone-Mediated Endocrine Regulation in the Indian Meal Moth Plodia interpunctella

Conifer Diterpene Resin Acids Disrupt Juvenile Hormone-Mediated Endocrine Regulation in the Indian Meal Moth Plodia interpunctella

  • J Chem Ecol. 2017 Jul;43(7):703-711. doi: 10.1007/s10886-017-0861-9.
Hyun-Woo Oh 1,  Chan-Seok Yun 2,  Jun Hyoung Jeon 2,  Ji-Ae Kim 3,  Doo-Sang Park 2,  Hyung Won Ryu 4,  Sei-Ryang Oh 4,  Hyuk-Hwan Song 5,  Yunhee Shin 6,  Chan Sik Jung 7,  Sang Woon Shin 8
Affiliations

Affiliations

  • 1 Core Facility Management Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea. hwoh@kribb.re.kr.
  • 2 Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, 580-185, Republic of Korea.
  • 3 Core Facility Management Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea.
  • 4 Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang, Chung-buk, 363-883, Republic of Korea.
  • 5 Agency for Korea National Food Cluster, Iksan, 507-749, Republic of Korea.
  • 6 Department of Integrative Biology, University of California, Berkley, CA, 94720, USA.
  • 7 Division of Forest Insect Pests and Disease, Korea Forest Research Institute, Seoul, 130-712, Republic of Korea.
  • 8 Core Facility Management Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea. shinwoongg@gmail.com.
PMID: 28674826 DOI: 10.1007/s10886-017-0861-9
Abstract

Diterpene resin acids (DRAs) are important components of oleoresin and greatly contribute to the defense strategies of conifers against herbivorous insects. In the present study, we determined that DRAs function as insect juvenile hormone (JH) antagonists that interfere with the juvenile hormone-mediated binding of the JH receptor Methoprene-tolerant (Met) and steroid receptor coactivator (Src). Using a yeast two-hybrid system transformed with Met and Src from the Indian meal moth Plodia interpunctella, we tested the interfering activity of 3704 plant extracts against JH III-mediated Met-SRC binding. Plant extracts from conifers, especially members of the Pinaceae, exhibited strong interfering activity, and four active interfering DRAs (7α-dehydroabietic acid, 7-oxodehydroabietic acid, dehydroabietic acid, and sandaracopimaric acid) were isolated from roots of the Japanese pine Pinus densiflora. The four isolated DRAs, along with abietic acid, disrupted the juvenile hormone-mediated binding of P. interpunctella Met and Src, although only 7-oxodehydroabietic acid disrupted larval development. These results demonstrate that DRAs may play a defensive role against herbivorous insects via insect endocrine-disrupting activity.

Keywords

Diterpene resin acid; Herbivore; Insect development; Juvenile hormone; Juvenile hormone antagonist; Plant defense; Terpenes.

Figures
Products