2. Academic Validation
  3. Molecular identification and phylogenetic analysis of human Trichostrongylus species from an endemic area of Iran

Molecular identification and phylogenetic analysis of human Trichostrongylus species from an endemic area of Iran

  • Acta Trop. 2017 Dec;176:293-299. doi: 10.1016/j.actatropica.2017.07.001.
Meysam Sharifdini 1,  Sedigheh Derakhshani 2,  Safar Ali Alizadeh 3,  Laleh Ghanbarzadeh 2,  Hamed Mirjalali 4,  Iraj Mobedi 5,  Mehrzad Saraei 6
Affiliations

Affiliations

  • 1 Department of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
  • 2 Department of Parasitology and Mycology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
  • 3 Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
  • 4 Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • 5 Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  • 6 Department of Parasitology and Mycology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran; Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran. Electronic address: msaraei@qums.ac.ir.
PMID: 28760481 DOI: 10.1016/j.actatropica.2017.07.001
Abstract

Human infections with Trichostrongylus species have been reported in most parts of Iran. The aim of this study was the identification, molecular characterization and phylogenetic analysis of human Trichostrongylus species based on ITS2 region of ribosomal DNA from Guilan Province, northern Iran. Stool samples were collected from rural inhabitants and examined by formalin-ether concentration and agar plate culture techniques. After anthelmintic treatment, male adult worms were collected from five infected cases. Genomic DNA was extracted from one male worm of each species in every treated individual and one filariform larva isolated from each case. PCR amplification of ITS2-rDNA region was performed and the products were sequenced. Among 1508 individuals, 46 (3.05%) were found infected with Trichostrongylus species using parasitological methods. Male worms of T. colubriformis, T. vitrinus and T. longispicularis were expelled from five patients after treatment. Out of 41 filariform larvae, 40 were T. colubriformis, and the other one was T. axei. Phylogenetic analysis showed that each species was placed together with reference sequences submitted to GenBank database. Intra-species similarity for all species obtained in the current study was 100%. T. colubriformis was found to be probably the most common species in this region of Iran. For the first time, the authors of the present study report the occurrence of natural human Infection by T. longispicularis in the world. Therefore, the number of Trichostrongylus species infecting human in Iran now increased to ten.

Keywords

Guilan; Human; ITS2-rDNA region; Iran; PCR; Phylogenetic analysis; Trichostrongylus.

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