2. Academic Validation
  3. Biphenyloxy-alkyl-piperidine and azepane derivatives as histamine H3 receptor ligands

Biphenyloxy-alkyl-piperidine and azepane derivatives as histamine H3 receptor ligands

  • Bioorg Med Chem. 2017 Oct 15;25(20):5341-5354. doi: 10.1016/j.bmc.2017.07.058.
Dorota Łażewska 1,  Maria Kaleta 2,  J Stephan Schwed 3,  Tadeusz Karcz 2,  Szczepan Mogilski 4,  Gniewomir Latacz 2,  Agnieszka Olejarz 2,  Agata Siwek 5,  Monika Kubacka 4,  Annamaria Lubelska 2,  Ewelina Honkisz 2,  Jadwiga Handzlik 2,  Barbara Filipek 4,  Holger Stark 3,  Katarzyna Kieć-Kononowicz 6
Affiliations

Affiliations

  • 1 Department of Technology and Biotechnology of Drugs, Faculty of Pharmacy, Jagiellonian University Medical College, ul. Medyczna 9, 30-688 Kraków, Poland. Electronic address: dlazewska@cm-uj.krakow.pl.
  • 2 Department of Technology and Biotechnology of Drugs, Faculty of Pharmacy, Jagiellonian University Medical College, ul. Medyczna 9, 30-688 Kraków, Poland.
  • 3 Institute of Pharmaceutical and Medicinal Chemistry, Heinrich-Heine-University Düsseldorf, Universitätsstr. 1, 40225 Düsseldorf, Germany.
  • 4 Department of Pharmacodynamic, Faculty of Pharmacy, Jagiellonian University Medical College, ul. Medyczna 9, 30-688 Kraków, Poland.
  • 5 Department of Pharmacobiology, Faculty of Pharmacy, Jagiellonian University Medical College, ul. Medyczna 9, 30-688 Kraków, Poland.
  • 6 Department of Technology and Biotechnology of Drugs, Faculty of Pharmacy, Jagiellonian University Medical College, ul. Medyczna 9, 30-688 Kraków, Poland. Electronic address: mfkonono@cyf-kr.edu.pl.
PMID: 28797771 DOI: 10.1016/j.bmc.2017.07.058
Abstract

Novel biphenyloxy-alkyl derivatives of piperidine and azepane were synthesized and evaluated for their binding properties at the human histamine H3 receptor. Two series of compounds were obtained with a meta- and a para-biphenyl moiety. The alkyl chain spacer contained five and six carbon atoms. The highest affinity among all compounds was shown by 1-(6-(3-phenylphenoxy)hexyl)azepane (13) with a Ki value of 18nM. Two para-biphenyl derivatives, 1-(5-(4-phenylphenoxy)pentyl)piperidine (14; Ki=25nM) and 1-(5-(4-phenylphenoxy)pentyl)azepane (16; Ki=34nM), classified as antagonists in a cAMP accumulation assay (IC50=4 and 9nM, respectively), were studied in detail. Compounds 14 and 16 blocked RAMH-induced dipsogenia in rats (ED50 of 2.72mg/kg and 1.75mg/kg respectively), and showed high selectivity (hH4R vs hH3R>600-fold) and low toxicity (hERG inhibition: IC50>1.70µM; hepatotoxicity IC50>12.5µM; non-mutagenic up to 10µM). Furthermore, the metabolic stability was evaluated in vitro on human liver microsomes (HLMs) and/or rat liver microsomes (RLMs). Metabolites produced were analyzed and tentatively identified by UPLC-MS techniques. The results demonstrated easy hydroxylation of the biphenyl ring.

Figures