2. Academic Validation
  3. BiHC, a T-Cell-Engaging Bispecific Recombinant Antibody, Has Potent Cytotoxic Activity Against Her2 Tumor Cells

BiHC, a T-Cell-Engaging Bispecific Recombinant Antibody, Has Potent Cytotoxic Activity Against Her2 Tumor Cells

  • Transl Oncol. 2017 Oct;10(5):780-785. doi: 10.1016/j.tranon.2017.07.003.
Jieyu Xing 1,  Limin Lin 2,  Jing Li 3,  Jiayu Liu 4,  Changhua Zhou 5,  Haitao Pan 6,  Rui Shu 7,  Bin Dong 8,  Donglin Cao 9,  Qing Li 10,  Zhong Wang 11
Affiliations

Affiliations

  • 1 School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, China 510006; Center for Cellular & Structural Biology, Sun Yat-Sen University, Guangzhou, China 510006. Electronic address: xingjy5@mail2.sysu.edu.cn.
  • 2 School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, China 510006; Center for Cellular & Structural Biology, Sun Yat-Sen University, Guangzhou, China 510006. Electronic address: linlm3@mail2.sysu.edu.cn.
  • 3 School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, China 510006; Center for Cellular & Structural Biology, Sun Yat-Sen University, Guangzhou, China 510006. Electronic address: lijing236@mail2.sysu.edu.cn.
  • 4 School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, China 510006; Center for Cellular & Structural Biology, Sun Yat-Sen University, Guangzhou, China 510006. Electronic address: liujy53@mail2.sysu.edu.cn.
  • 5 School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, China 510006; Center for Cellular & Structural Biology, Sun Yat-Sen University, Guangzhou, China 510006. Electronic address: zhouchh5@mail2.sysu.edu.cn.
  • 6 School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, China 510006; Center for Cellular & Structural Biology, Sun Yat-Sen University, Guangzhou, China 510006. Electronic address: panht3@mail2.sysu.edu.cn.
  • 7 Ying Rui Inc., Guangzhou, Guangdong, China 510009. Electronic address: 13928799130@163.com.
  • 8 School of Biosciences and Biopharmaceutics, Guangdong pharmaceutical University, Guangzhou, Guangdong, China 510009. Electronic address: daviddong99@163.com.
  • 9 Department of Laboratory Medicine, Guangdong No. 2 Provincial People's Hospital, Guangzhou, China 510317. Electronic address: caodl@126.com.
  • 10 School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, China 510006; Center for Cellular & Structural Biology, Sun Yat-Sen University, Guangzhou, China 510006. Electronic address: liqing66@mail.sysu.edu.cn.
  • 11 School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, China 510006; Center for Cellular & Structural Biology, Sun Yat-Sen University, Guangzhou, China 510006. Electronic address: wangzh357@mail.sysu.edu.cn.
PMID: 28797938 DOI: 10.1016/j.tranon.2017.07.003
Abstract

Among different Cancer Immunotherapy approaches, bispecific Antibodies (BsAbs) are of great interest due to their ability to recruit immune cells to kill tumor cells directly. Various BsAbs against Her2 tumor cells have been proposed with potent cytotoxic activities. However, most of these formats require extensive processing to obtain heterodimeric bispecific Antibodies. In this study, we describe a bispecific antibody, BiHC (bispecific Her2-CD3 antibody), constructed with a single-domain anti-Her2 and a single-chain Fv (variable fragment) of anti-CD3 in an IgG-like format. In contrast to most IgG-like BsAbs, the two arms in BiHC have different molecular weights, making it easier to separate hetero- or homodimers. BiHC can be expressed in Escherichia coli and purified via Protein A affinity chromatography. The purified BiHC can recruit T cells and induce specific cytotoxicity of Her2-expressing tumor cells in vitro. The BiHC can also efficiently inhibit the tumor growth in vivo. Thus, BiHC is a promising candidate for the treatment of Her2-positive cancers.

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Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-152169
    Her2-CD3 Bispecific Antibody