2. Academic Validation
  3. Purification of functional reprogramming factors in mammalian cell using FLAG -Tag

Purification of functional reprogramming factors in mammalian cell using FLAG -Tag

  • Biochem Biophys Res Commun. 2017 Oct 14;492(2):154-160. doi: 10.1016/j.bbrc.2017.08.028.
Min-Joon Han 1,  Ha Ram Kim 2,  Carla O'Reilly 2,  Chun-Hyung Kim 3
Affiliations

Affiliations

  • 1 St. Jude Children's Research Hospital, Department of Hematology, 262 Danny Thomas Place, Memphis, TN 38105, USA; Molecular Neurobiology Laboratory, Department of Psychiatry, McLean Hospital/Harvard Medical School, 115 Mill Street, Belmont, MA 02478, USA. Electronic address: Min-Joon.Han@stjude.org.
  • 2 St. Jude Children's Research Hospital, Department of Hematology, 262 Danny Thomas Place, Memphis, TN 38105, USA.
  • 3 Molecular Neurobiology Laboratory, Department of Psychiatry, McLean Hospital/Harvard Medical School, 115 Mill Street, Belmont, MA 02478, USA; PAEAN Biotechnology, Republic of Korea. Electronic address: chkim@paeanbio.com.
PMID: 28802578 DOI: 10.1016/j.bbrc.2017.08.028
Abstract

Induced pluripotent stem cells (iPSCs) technology is a method for generating pluripotent stem cells in vitro from fully differentiated cells such as fibroblast cells. The potential applications of iPSC technology in cell therapy and disease modeling could influence current medical practices. Despite current advances in iPSC technology, many patient-derived reprogrammed cells are not suitable for clinical trial because most protocols rely on virus-based techniques, which pose the risk of integration of the viral genome into the chromosomes. Therefore, non-viral methods such as mRNA and protein-based reprogramming are promising alternatives when generating clinically safe iPSCs. In a previous study, we generated human iPSCs using cell extracts with cell penetration peptide (CPP) for the delivery of reprogramming proteins [Kim et al. Cell Stem Cells, 2009]. In here, we show that the expression of reprogramming factors in mammalian cells and subsequent purification of these factors by FLAG-Tag could reprogram fibroblasts into iPSCs.

Keywords

Induced pluripotent stem cell; Protein delivery; Reprogramming.

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