2. Academic Validation
  3. TRIM23 mediates virus-induced autophagy via activation of TBK1

TRIM23 mediates virus-induced autophagy via activation of TBK1

  • Nat Microbiol. 2017 Nov;2(11):1543-1557. doi: 10.1038/s41564-017-0017-2.
Konstantin M J Sparrer 1,  Sebastian Gableske 1,  Matthew A Zurenski 1,  Zachary M Parker 1,  Florian Full 1,  Gavin J Baumgart 1,  Jiro Kato 2,  Gustavo Pacheco-Rodriguez 2,  Chengyu Liang 3,  Owen Pornillos 4,  Joel Moss 2,  Martha Vaughan 2,  Michaela U Gack 5
Affiliations

Affiliations

  • 1 Department of Microbiology, The University of Chicago, Chicago, IL, 60637, USA.
  • 2 Cardiovascular and Pulmonary Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
  • 3 Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA, 90033, USA.
  • 4 Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA, 22908, USA.
  • 5 Department of Microbiology, The University of Chicago, Chicago, IL, 60637, USA. mgack@uchicago.edu.
PMID: 28871090 DOI: 10.1038/s41564-017-0017-2
Abstract

Autophagy and interferon (IFN)-mediated innate immunity are critical Antiviral defence mechanisms, and recent evidence indicated that tripartite motif (TRIM) proteins are important regulators of both processes. Although the role of TRIM proteins in modulating Antiviral cytokine responses has been well established, much less is known about their involvement in Autophagy in response to different viral pathogens. Through a targeted RNAi screen examining the relevance of selected TRIM proteins in Autophagy induced by herpes simplex virus 1 (HSV-1), encephalomyocarditis virus (EMCV) and influenza A virus (IAV), we identified several TRIM proteins that regulate Autophagy in a virus-species-specific manner, as well as a few TRIM proteins that were essential for Autophagy triggered by all three viruses and rapamycin, among them TRIM23. TRIM23 was critical for autophagy-mediated restriction of multiple viruses, and this activity was dependent on both its RING E3 Ligase and ADP-ribosylation factor (ARF) GTPase activity. Mechanistic studies revealed that unconventional K27-linked auto-ubiquitination of the ARF domain is essential for the GTP hydrolysis activity of TRIM23 and activation of TANK-binding kinase 1 (TBK1) by facilitating its dimerization and ability to phosphorylate the selective Autophagy receptor p62. Our work identifies the TRIM23-TBK1-p62 axis as a key component of selective Autophagy and further reveals a role for K27-linked ubiquitination in GTPase-dependent TBK1 activation.

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