2. Academic Validation
  3. TMEM106a is a Novel Tumor Suppressor in Human Renal Cancer

TMEM106a is a Novel Tumor Suppressor in Human Renal Cancer

  • Kidney Blood Press Res. 2017;42(5):853-864. doi: 10.1159/000484495.
Chenglong Wu 1,  Juan Xu 2,  Han Wang 3,  Jinghua Zhang 2,  Jianhua Zhong 2,  Xiaowen Zou 2,  Yan Chen 2,  Gang Yang 4,  Yuantang Zhong 4,  Daihuan Lai 2,  Xuyi Li 2,  Aifa Tang 2
Affiliations

Affiliations

  • 1 Shenzhen Second People's Hospital, Guangzhou Medical University, Shenzhen, China.
  • 2 Department of Urinary Surgery, Shenzhen Second People's Hospital, the First Affiliated Hospital of Shenzhen University, Shenzhen, China.
  • 3 Clinical Medicine College of Anhui Medical University, Hefei, China.
  • 4 Guangzhou medical university, Guangzhou, China.
PMID: 29131025 DOI: 10.1159/000484495
Abstract

Background/aims: In recent years the diagnosis and management of renal Cancer has changed greatly, although the mechanism is still elusive. TMEM106a is a conserved type II transmembrane protein which is a key factor to regulate macrophage activation. Its inactivation in gastric Cancer is frequently observed to be associated with poor prognosis. The role of TMEM106a in renal Cancer remained unclear.

Methods: TMEM106a expression profiling was performed in a panel of renal Cancer cell lines and primary renal tissue cells. Then TMEM106a was overexpressed by a viral system in a renal Cancer cell line with low level of TMEM106a. This stable cell line was assessed in multiple cell growth and migration assays. The results from TMEM106a overexpressing cell line were then confirmed with primary renal cells by siRNA knockdown of TMEM106a.

Results: TMEM106a expression level was reduced in renal Cancer cells compared to normal primary renal cells. Restoration of TMEM106a expression in TMEM106a-low renal Cancer cells resulted in attenuated proliferation, reduced cell migration and enhanced Caspase 3 dependent Apoptosis compared to control cells. TMEM106a knockdown in primary renal cells led to increased colony formation compared to the control cells with scrambled siRNA transfection.

Conclusion: TMEM106a is a novel tumor suppressor in renal Cancer.

Keywords

Apoptosis; Cell migration; Proliferation; Renal cancer; TMEM106a.

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