2. Academic Validation
  3. 5-(Hydroxymethyl)uracil and -cytosine as potential epigenetic marks enhancing or inhibiting transcription with bacterial RNA polymerase

5-(Hydroxymethyl)uracil and -cytosine as potential epigenetic marks enhancing or inhibiting transcription with bacterial RNA polymerase

  • Chem Commun (Camb). 2017 Dec 12;53(99):13253-13255. doi: 10.1039/c7cc08053k.
Martina Janoušková 1,  Zuzana Vaníková,  Fabrizia Nici,  Soňa Boháčová,  Dragana Vítovská,  Hana Šanderová,  Michal Hocek,  Libor Krásný
Affiliations

Affiliation

  • 1 Dept. of Molecular Genetics of Bacteria, Institute of Microbiology of the Czech Academy of Sciences, CZ-14220 Prague 4, Czech Republic. krasny@biomed.cas.cz.
PMID: 29184924 DOI: 10.1039/c7cc08053k
Abstract

DNA templates containing 5-hydroxymethyluracil or 5-hydroxymethylcytosine were used in an in vitro transcription assay with RNA polymerase from Escherichia coli. A strong enhancement of transcription was observed from DNA containing the Pveg promoter whereas a decrease was observed from DNA containing the rrnB P1 promoter, suggesting that they may act as epigenetic marks.

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