1. Academic Validation
  2. Ultrasensitive electrochemical detection of poly (ADP-ribose) polymerase-1 via polyaniline deposition

Ultrasensitive electrochemical detection of poly (ADP-ribose) polymerase-1 via polyaniline deposition

  • Talanta. 2018 Apr 1;180:127-132. doi: 10.1016/j.talanta.2017.11.072.
Yong Liu 1 Jiahui Fan 2 Li Shangguan 2 Yuanjian Liu 2 Yuanqing Wei 2 Wei Wei 3 Songqin Liu 2
Affiliations

Affiliations

  • 1 Henan Key Laboratory of Polyoxometalat, Institute of Fine Chemistry and Engineering, College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, PR China.
  • 2 Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, PR China.
  • 3 Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, PR China. Electronic address: weiw@seu.edu.cn.
Abstract

Recent findings have thrust poly ADP (ADP: adenosine diphosphate)-ribose polymerase-1 (PARP-1) into the limelight as potential chemotherapeutic target because it is closely related to the development of tumor. So, studies on its detection and inhibitors evaluation have attracted more attention. It is interesting that poly (ADP-ribose) (PAR), the catalytic product of PARP-1 in the existence of nicotinamide adenine dinucleotide (NAD+), possess twice charge density of DNA strands. PAR contain 200 units, i.e., about 400bp bases, and multiple branched strands. So, plentiful negative charges on PAR supplied exquisite environment for PANI deposition, which was triggered by horseradish peroxidase (HRP). Because of the unique electrochemical property of PANI, ultrasensitive electrochemical detection of PARP-1 was proposed. Under optimum conditions, DPV intensity linearly increased with the increment of PARP-1 in the range of 0.005-1.0 U. The detection limit was 0.002 U, which was comparable or more sensitive than that obtained from previously reported strategies.

Keywords

Electrochemistry; Poly (ADP-ribose); Poly (adenosine diphosphate)-ribose polymerase; Polyaniline.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-10617A
    99.97%, PARP Inhibitor