2. Academic Validation
  3. Targeting KRAS Mutant Cancers with a Covalent G12C-Specific Inhibitor

Targeting KRAS Mutant Cancers with a Covalent G12C-Specific Inhibitor

  • Cell. 2018 Jan 25;172(3):578-589.e17. doi: 10.1016/j.cell.2018.01.006.
Matthew R Janes 1,  Jingchuan Zhang 1,  Lian-Sheng Li 1,  Rasmus Hansen 1,  Ulf Peters 1,  Xin Guo 1,  Yuching Chen 1,  Anjali Babbar 1,  Sarah J Firdaus 1,  Levan Darjania 1,  Jun Feng 1,  Jeffrey H Chen 1,  Shuangwei Li 1,  Shisheng Li 1,  Yun O Long 1,  Carol Thach 1,  Yuan Liu 1,  Ata Zarieh 1,  Tess Ely 1,  Jeff M Kucharski 1,  Linda V Kessler 1,  Tao Wu 1,  Ke Yu 1,  Yi Wang 1,  Yvonne Yao 1,  Xiaohu Deng 1,  Patrick P Zarrinkar 1,  Dirk Brehmer 2,  Dashyant Dhanak 3,  Matthew V Lorenzi 3,  Dana Hu-Lowe 1,  Matthew P Patricelli 1,  Pingda Ren 4,  Yi Liu 5
Affiliations

Affiliations

  • 1 Wellspring Biosciences, San Diego, CA, USA.
  • 2 Oncology Discovery, Janssen Research & Development, Beerse, Belgium.
  • 3 Janssen Research & Development, Spring House, PA, USA.
  • 4 Wellspring Biosciences, San Diego, CA, USA; Kura Oncology, San Diego, CA, USA.
  • 5 Wellspring Biosciences, San Diego, CA, USA; Kura Oncology, San Diego, CA, USA. Electronic address: yi@kuraoncology.com.
PMID: 29373830 DOI: 10.1016/j.cell.2018.01.006
Abstract

KRASG12C was recently identified to be potentially druggable by allele-specific covalent targeting of Cys-12 in vicinity to an inducible allosteric switch II pocket (S-IIP). Success of this approach requires active cycling of KRASG12C between its active-GTP and inactive-GDP conformations as accessibility of the S-IIP is restricted only to the GDP-bound state. This strategy proved feasible for inhibiting mutant KRAS in vitro; however, it is uncertain whether this approach would translate to in vivo. Here, we describe structure-based design and identification of ARS-1620, a covalent compound with high potency and selectivity for KRASG12C. ARS-1620 achieves rapid and sustained in vivo target occupancy to induce tumor regression. We use ARS-1620 to dissect oncogenic KRAS dependency and demonstrate that monolayer culture formats significantly underestimate KRAS dependency in vivo. This study provides in vivo evidence that mutant KRAS can be selectively targeted and reveals ARS-1620 as representing a new generation of KRASG12C-specific inhibitors with promising therapeutic potential.

Keywords

3D culture; ARS-1620; G12C; KRAS; NSCLC; RAS; addiction; dependence; oncogene.

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