2. Academic Validation
  3. PKM2 knockdown influences SREBP activation and lipid synthesis in bovine mammary-gland epithelial MAC-T cells

PKM2 knockdown influences SREBP activation and lipid synthesis in bovine mammary-gland epithelial MAC-T cells

  • Biotechnol Lett. 2018 Apr;40(4):641-648. doi: 10.1007/s10529-018-2518-3.
Bing-Qian Su 1,  Ying-Qian Han 1,  Shuang-Shuang Fan 1,  Sheng-Li Ming 1,  Bo Wan 1,  Wei-Fei Lu 1,  Bei-Bei Chu 1,  Guo-Yu Yang 1,  Jiang Wang 2
Affiliations

Affiliations

  • 1 College of Animal Sciences and Veterinary Medicine, Henan Agricultural University, No. 95, Wenhua Road, Zhengzhou, 450002, Henan, People's Republic of China.
  • 2 College of Animal Sciences and Veterinary Medicine, Henan Agricultural University, No. 95, Wenhua Road, Zhengzhou, 450002, Henan, People's Republic of China. wangjiang_hau@126.com.
PMID: 29383471 DOI: 10.1007/s10529-018-2518-3
Abstract

Objective: The purpose of the article is to evaluate the changes in lipid metabolism in bovine mammary-gland epithelial MAC-T cells after PKM2 knockdown.

Results: MAC-T cells stably expressing low levels of PKM2 were established with lentivirus-mediated small hairpin RNA. Although the knockdown of PKM2 had no effect on MAC-T cell growth, the reduced expression of PKM2 attenuated the mRNA and protein expression of key Enzymes involved in sterol synthesis through the SREBP pathway.

Conclusions: The downregulation of PKM2 significantly influenced lipid synthesis in bovine mammary-gland epithelial MAC-T cells. These findings extend our understanding of the crosstalk between glycolysis and lipid metabolism in bovine mammary-gland epithelial cells.

Keywords

Bovine mammary-gland epithelial cells; Lipid synthesis; PKM2; SREBP.

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