2. Academic Validation
  3. The interaction of selenoprotein F (SELENOF) with retinol dehydrogenase 11 (RDH11) implied a role of SELENOF in vitamin A metabolism

The interaction of selenoprotein F (SELENOF) with retinol dehydrogenase 11 (RDH11) implied a role of SELENOF in vitamin A metabolism

  • Nutr Metab (Lond). 2018 Jan 22;15:7. doi: 10.1186/s12986-017-0235-x.
Jing Tian 1,  Jiapan Liu 1,  Jieqiong Li 2,  Jingxin Zheng 3,  Lifang Chen 4,  Yujuan Wang 1,  Qiong Liu 1,  Jiazuan Ni 1
Affiliations

Affiliations

  • 1 1College of Life Sciences and Oceanography, Shenzhen Key Laboratory of Marine Bioresources and Eco-environmental Science, Shenzhen University, Shenzhen, 518060 China.
  • 2 2College of Life Sciences and Oceanography, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University, Shenzhen, 518060 China.
  • 3 3College of Life Sciences and Oceanography, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Shenzhen University, Shenzhen, 518060 China.
  • 4 4Department of Neurology, Shenzhen University 1st Affiliated Hospital, Shenzhen Second People's Hospital, Sungang West Road, Shenzhen, China.
PMID: 29410696 DOI: 10.1186/s12986-017-0235-x
Abstract

Background: Selenoprotein F (SELENOF, was named as 15-kDa selenoprotein) has been reported to play important roles in oxidative stress, endoplasmic reticulum (ER) stress and carcinogenesis. However, the biological function of SELENOF is still unclear.

Methods: A yeast two-hybrid system was used to screen the interactive protein of SELENOF in a human fetal brain cDNA library. The interaction between SELENOF and interactive protein was validated by fluorescence resonance energy transfer (FRET), co-immunoprecipitation (co-IP) and pull-down assays. The production of retinol was detected by high performance liquid chromatograph (HPLC).

Results: Retinol dehydrogenase 11 (RDH11) was found to interact with SELENOF. RDH11 is an Enzyme for the reduction of all-trans-retinaldehyde to all-trans-retinol (vitamin A). The production of retinol was decreased by SELENOF overexpression, resulting in more retinaldehyde.

Conclusions: SELENOF interacts with RDH11 and blocks its Enzyme activity to reduce all-trans-retinaldehyde.

Keywords

Co-immunoprecipitation (co-IP); Fluorescence resonance energy transfer (FRET); Protein-protein interaction; Pull-down; Retinaldehyde; Retinol (vitamin a); Retinol dehydrogenase 11 (RDH11); SELENOF (Seleonoprotein F); Yeast two hybrid system.

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