2. Academic Validation
  3. AUX1-mediated root hair auxin influx governs SCFTIR1/AFB-type Ca2+ signaling

AUX1-mediated root hair auxin influx governs SCFTIR1/AFB-type Ca2+ signaling

  • Nat Commun. 2018 Mar 21;9(1):1174. doi: 10.1038/s41467-018-03582-5.
Julian Dindas 1,  Sönke Scherzer 1,  M Rob G Roelfsema 1,  Katharina von Meyer 1,  Heike M Müller 1,  K A S Al-Rasheid 2,  Klaus Palme 3,  Petra Dietrich 4,  Dirk Becker 1,  Malcolm J Bennett 5,  Rainer Hedrich 6
Affiliations

Affiliations

  • 1 Institute for Molecular Plant Physiology and Biophysics, University of Würzburg, 97082, Würzburg, Germany.
  • 2 College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
  • 3 Institute of Biology II/Molecular Plant Physiology, Faculty of Biology, BIOSS Centre for Biological Signaling Studies, Centre for Biological Systems Analysis, 79104, Freiburg, Germany.
  • 4 Molecular Plant Physiology, Department of Biology, University of Erlangen-Nürnberg, Staudtstrasse 5, 91058, Erlangen, Germany.
  • 5 Centre for Plant Integrative Biology, Plant & Crop Sciences, School of Biosciences, University of Nottingham, Nottingham, LE12 3RD, UK.
  • 6 Institute for Molecular Plant Physiology and Biophysics, University of Würzburg, 97082, Würzburg, Germany. hedrich@botanik.uni-wuerzburg.de.
PMID: 29563504 DOI: 10.1038/s41467-018-03582-5
Abstract

Auxin is a key regulator of plant growth and development, but the causal relationship between hormone transport and root responses remains unresolved. Here we describe Auxin uptake, together with early steps in signaling, in Arabidopsis root hairs. Using intracellular microelectrodes we show membrane depolarization, in response to IAA in a concentration- and pH-dependent manner. This depolarization is strongly impaired in aux1 mutants, indicating that AUX1 is the major transporter for Auxin uptake in root hairs. Local intracellular Auxin application triggers CA2+ signals that propagate as long-distance waves between root cells and modulate their Auxin responses. AUX1-mediated IAA transport, as well as IAA- triggered calcium signals, are blocked by treatment with the SCFTIR1/AFB - inhibitor auxinole. Further, they are strongly reduced in the tir1afb2afb3 and the cngc14 mutant. Our study reveals that the AUX1 transporter, the SCFTIR1/AFB receptor and the CNGC14 CA2+ channel, mediate fast Auxin signaling in roots.

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Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-111444
    99.77%, SCF(TIR1/AFB) Antagonist