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  3. Gynosaponin TN-1 producing from the enzymatic conversion of gypenoside XLVI by naringinase and its cytotoxicity on hepatoma cell lines

Gynosaponin TN-1 producing from the enzymatic conversion of gypenoside XLVI by naringinase and its cytotoxicity on hepatoma cell lines

  • Food Chem Toxicol. 2018 Sep;119:161-168. doi: 10.1016/j.fct.2018.05.007.
Yi Zheng 1,  Zhizhong Zheng 2,  Yanlin Ming 3,  Mengshi Lin 4,  Lianghua Chen 5,  Wen Huang 6,  Jianbo Xiao 7,  Hetong Lin 8
Affiliations

Affiliations

  • 1 College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China; Key Laboratory of Xiamen City for Plant Introduction & Quarantine and Plant Derived Product, Xiamen Overseas Chinese Subtropical Plant Introduction Garden, Xiamen, Fujian 361002, China.
  • 2 Key Laboratory of Xiamen City for Plant Introduction & Quarantine and Plant Derived Product, Xiamen Overseas Chinese Subtropical Plant Introduction Garden, Xiamen, Fujian 361002, China; Key Laboratory of Fujian Province for Physiology and Biochemistry of Subtropical Plant, Fujian Institute of Subtropical Botany, Xiamen, Fujian, 361006, China.
  • 3 College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China; Key Laboratory of Xiamen City for Plant Introduction & Quarantine and Plant Derived Product, Xiamen Overseas Chinese Subtropical Plant Introduction Garden, Xiamen, Fujian 361002, China; Key Laboratory of Fujian Province for Physiology and Biochemistry of Subtropical Plant, Fujian Institute of Subtropical Botany, Xiamen, Fujian, 361006, China. Electronic address: xmyanlin@gmail.com.
  • 4 Food Science Program, Division of Food System & Bioengineering, University of Missouri, Columbia, MO 65211-5160, USA.
  • 5 College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China; Key Laboratory of Fujian Province for Physiology and Biochemistry of Subtropical Plant, Fujian Institute of Subtropical Botany, Xiamen, Fujian, 361006, China.
  • 6 Key Laboratory of Fujian Province for Physiology and Biochemistry of Subtropical Plant, Fujian Institute of Subtropical Botany, Xiamen, Fujian, 361006, China.
  • 7 College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China. Electronic address: jianboxiao@yahoo.com.
  • 8 College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China. Electronic address: hetonglin@163.com.
PMID: 29751078 DOI: 10.1016/j.fct.2018.05.007
Abstract

Gypenoside XLVI (gyp XLVI) is one of the major dammarane-type triterpenoid saponins from Gynostamma pentaphallum with glucosyls at C-3 and C-20 positions, which may constrain its bioactivities. The enzymatic conversion of gyp XLVI by naringinase, and the cytotoxicity of enzymolysis product on SMMC7721 and Bel7402 hepatoma cells were investigated. The results showed that gynosaponin TN-1 (gyp TN-1) was produced from the enzymatic conversion of gyp XLVI by naringinase. The optimum enzymolysis conditions were pH 4.2, 47.3 °C, and 16 h, with a yield of 73.44 ± 6.52% for gyp TN-1. In addition, gyp TN-1 exhibited higher inhibitory activities on SMMC7721 and Bel7402 hepatoma cells than gyp XLVI. Results from methyl thiazolyl tetrazolium (MTT) assay and acridine orange (AO)/ethidium bromide (EB) double staining were highly consistent. These results demonstrated that enzymatic conversion could be a promising method for producing gyp TN-1 from the biotransformation of gyp XLVI and the preparation of gyp TN-1 might provide a reference for the acquisition of novel Anticancer drugs.

Keywords

Biotransformation; Enzymatic conversion; Gynosaponin TN-1; Gypenoside XLVI; Hepatoma cell lines; Naringinase.

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