1. Academic Validation
  2. The NK1 receptor antagonist NKP608 inhibits proliferation of human colorectal cancer cells via Wnt signaling pathway

The NK1 receptor antagonist NKP608 inhibits proliferation of human colorectal cancer cells via Wnt signaling pathway

  • Biol Res. 2018 May 30;51(1):14. doi: 10.1186/s40659-018-0163-x.
Xiao-Ling Niu 1 Jian-Feng Hou 2 Jing-Xiang Li 3
Affiliations

Affiliations

  • 1 Department of Traditional Chinese Medicine, Shanghai Pudong New Area Zhoupu Hospital, Shanghai, 201318, People's Republic of China.
  • 2 Department of Hepatobiliary Surgery, The First Hospital of Yulin City, Yulin, 719000, Shaanxi, People's Republic of China.
  • 3 Anorectal Department, Dongzhimen Hospital, Beijing University of Chinese Medicine, Hai Yun Cang on the 5th Zip, Dongcheng District, Beijing, 100700, People's Republic of China. ljxsir@126.com.
Abstract

Background: Neurokinin1 (NK1) receptor has played a vital role in the development of tumor. However, NKP608 as a NK1 receptor antagonist whether has the effect of the resistance of colorectal Cancer is still unclear. Thereby, in this study, we investigated the role of NKP608 on human colorectal Cancer and explored the underlying mechanism.

Methods: The cell proliferation of colorectal Cancer cells was detected by cell counting kit-8 (CCK8) assay, cell migration and invasion were assessed by transwell assay, the apoptotic ratio of cells was assessed by Annexin V-fluorescein isothiocyanate/propidium iodide stained and flow cytometry. The involvement of molecular mechanisms was examined by western blot.

Results: In this study, we found that NKP608 inhibited the proliferation, migration/invasion of HCT116 cells. In addition, NKP608 reduced expressions of Wnt-3a, β-catenin, Cyclin D1, and (vascular endothelial growth factor) VEGF while induced expression of E-Cadherin. Furthermore, flow cytometry analyzed that NKP608 induced Apoptosis of HCT116 cells, consistently, western blotting detecting of apoptosis-related proteins revealed that NKP608 downregulated Bcl-2 while upregulated Bax and Active-Caspase-3.

Conclusions: Taken together, our results demonstrated that NKP608 inhibited colorectal Cancer cell proliferation, migration and invasion via suppressing the Wnt/β-catenin signaling pathway. Therefore, NKP608 might represent a promising therapeutic agent in the treatment of colorectal Cancer.

Keywords

Colorectal cancer; NKP608; Proliferation; Wnt signaling pathway.

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