2. Academic Validation
  3. Identification of a peptide for folate receptor alpha by phage display and its tumor targeting activity in ovary cancer xenograft

Identification of a peptide for folate receptor alpha by phage display and its tumor targeting activity in ovary cancer xenograft

  • Sci Rep. 2018 May 30;8(1):8426. doi: 10.1038/s41598-018-26683-z.
Lijun Xing 1,  Yifeng Xu 1,  Keyong Sun 1,  Hong Wang 1,  Fengguo Zhang 1,  Zhengpin Zhou 1,  Juan Zhang 1,  Fang Zhang 2,  Bilgen Caliskan 1,  Zheng Qiu 3,  Min Wang 4
Affiliations

Affiliations

  • 1 School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, P.R. China.
  • 2 Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, P.R. China.
  • 3 School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, P.R. China. qiuzheng@cpu.edu.cn.
  • 4 School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, P.R. China. minwang@cpu.edu.cn.
PMID: 29849110 DOI: 10.1038/s41598-018-26683-z
Abstract

The expression level of folate receptor alpha (FRα) is located highly rate in ovarian Cancer though it is remained absent in normal tissues. This highly tumor restricted expression profile makes FRα a promising target for tumor therapy and diagnosis. In this research we report a FRα binding peptide C7(Met-His-Thr-Ala-Pro-Gly-Trp-Gly-Tyr-Arg-Leu-Ser) discovered by phage display and this peptide showed specific binding to FRα expressing cells by cell ELISA and flow cytometry. Tumor targeting ability of C7 was proved in vivo by both phage homing experiment and fluorescence imaging. C7 can be internalized by SKOV3 cells and its affinity to FRα was determined by MST. The molecular recognition was revealed by structure modeling, suggesting its binding mode with FRα.

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