2. Academic Validation
  3. New Records of Potent In-Vitro Antidiabetic Properties of Dalbergia tonkinensis Heartwood and the Bioactivity-Guided Isolation of Active Compounds

New Records of Potent In-Vitro Antidiabetic Properties of Dalbergia tonkinensis Heartwood and the Bioactivity-Guided Isolation of Active Compounds

  • Molecules. 2018 Jun 29;23(7):1589. doi: 10.3390/molecules23071589.
Van Bon Nguyen 1 2,  San-Lang Wang 3 4,  Ngu Truong Nhan 5 6,  Thi Hanh Nguyen 7,  Nguyen Phuong Dai Nguyen 8,  Do Huu Nghi 9 10,  Nguyen Manh Cuong 11 12
Affiliations

Affiliations

  • 1 Institute of Research and Development, Duy Tan University, Da Nang 550000, Vietnam. bondhtn@gmail.com.
  • 2 Department of Science and Technology, Tay Nguyen University, Buon Ma Thuot City 630000, Vietnam. bondhtn@gmail.com.
  • 3 Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan. sabulo@mail.tku.edu.tw.
  • 4 Life Science Development Center, Tamkang University, New Taipei City 25137, Taiwan. sabulo@mail.tku.edu.tw.
  • 5 Department of Science and Technology, Tay Nguyen University, Buon Ma Thuot City 630000, Vietnam. ngutruongnhan@gmail.com.
  • 6 Graduate University of Science and Technology, Vietnam Academy of Science and Technology (VAST), Hanoi 122100, Vietnam. ngutruongnhan@gmail.com.
  • 7 Department of Science and Technology, Tay Nguyen University, Buon Ma Thuot City 630000, Vietnam. nguyenhanh2208.tn@gmail.com.
  • 8 Department of Science and Technology, Tay Nguyen University, Buon Ma Thuot City 630000, Vietnam. nguyendhtn@gmail.com.
  • 9 Graduate University of Science and Technology, Vietnam Academy of Science and Technology (VAST), Hanoi 122100, Vietnam. dohnghi@gmail.com.
  • 10 Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST), Hanoi 122100, Vietnam. dohnghi@gmail.com.
  • 11 Graduate University of Science and Technology, Vietnam Academy of Science and Technology (VAST), Hanoi 122100, Vietnam. nmcuong_inpc@yahoo.com.vn.
  • 12 Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST), Hanoi 122100, Vietnam. nmcuong_inpc@yahoo.com.vn.
PMID: 29966279 DOI: 10.3390/molecules23071589
Abstract

Alpha-glucosidase inhibitory activity has been commonly used for the evaluation of antidiabetic property in vitro. The aim of this study is to investigate and characterize Dalbergia tonkinensis as a potential source of antidiabetic compounds. The screening of the active parts used, such as trunk bark, heartwood, and the leaves of Dalbergia tonkinensis indicated that all these extracted parts used with methanol demonstrated potent α-glucosidase inhibitory activity. The in vitro antidiabetic property of Dalbergia tonkinensis was notably recorded for the first time and showed activity (EC50 = 0.17⁻0.78 mg/mL) comparable to those of reported potent herbal extracts (EC50 = 0.25⁻4.0 mg/mL) and higher activity than that of acarbose, a commercial antidiabetic drug (EC50 = 1.21 mg/mL). The stability tests revealed that the heartwood of Dalbergia tonkinensis extract (HDT) possesses high pH stability with relative activity in the range of 80⁻98%. Further bioassay-guided purification led to the isolation of 2 active compounds identified as sativanone and formononetin from the ethyl acetate fraction and water fraction of HDT, respectively. These α-glucosidase inhibitors (aGIs) show promising inhibition against various types of α-glucosidases. Remarkably, these inhibitors were determined as new mammalian aGIs, showing good effect on rat α-glucosidase. The results suggest that Dalbergia tonkinensis is a potent source of aGIs and suggest promise in being developed as functional food with antidiabetic efficacy. The results of this study also enrich our knowledge concerning current biological activity and constituents of Dalbergia tonkinensis species.

Keywords

Dalbergia tonkinensis; diabetes; formononetin; heartwood; inhibitors; sativanone.

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