2. Academic Validation
  3. (-)-Asarinin from the Roots of Asarum sieboldii Induces Apoptotic Cell Death via Caspase Activation in Human Ovarian Cancer Cells

(-)-Asarinin from the Roots of Asarum sieboldii Induces Apoptotic Cell Death via Caspase Activation in Human Ovarian Cancer Cells

  • Molecules. 2018 Jul 25;23(8):1849. doi: 10.3390/molecules23081849.
Miran Jeong 1,  Hye Mi Kim 2,  Jin Su Lee 3 4,  Jung-Hye Choi 5 6,  Dae Sik Jang 7 8
Affiliations

Affiliations

  • 1 College of Pharmacy, Kyung Hee University, Seoul 02447, Korea. jeongmiran@hanmail.net.
  • 2 College of Pharmacy, Kyung Hee University, Seoul 02447, Korea. hyemi586@gmail.com.
  • 3 College of Pharmacy, Kyung Hee University, Seoul 02447, Korea. lee2649318@naver.com.
  • 4 Department of Life & Nanopharmaceutical Sciences, Kyung Hee University, Seoul 02447, Korea. lee2649318@naver.com.
  • 5 College of Pharmacy, Kyung Hee University, Seoul 02447, Korea. jchoi@khu.ac.kr.
  • 6 Department of Life & Nanopharmaceutical Sciences, Kyung Hee University, Seoul 02447, Korea. jchoi@khu.ac.kr.
  • 7 College of Pharmacy, Kyung Hee University, Seoul 02447, Korea. dsjang@khu.ac.kr.
  • 8 Department of Life & Nanopharmaceutical Sciences, Kyung Hee University, Seoul 02447, Korea. dsjang@khu.ac.kr.
PMID: 30044423 DOI: 10.3390/molecules23081849
Abstract

Two tetrahydrofurofurano Lignans (1 and 2), four Phenylpropanoids (3⁻6), and two alkamides (7 and 8) were isolated from the EtOAc-soluble fraction of the roots of Asarum sieboldii. The chemical structures of the isolates were identified by analysis of spectroscopic data measurements, and by a comparison of their data with published values. The isolates (1, 2, 4⁻8) were evaluated for their cytotoxicity against human ovarian Cancer cells (A2780 and SKOV3) and immortalized ovarian surface epithelial cells (IOSE80PC) using a MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide) assay. Of the isolates, (-)-asarinin (1) exhibited the most potent cytotoxicity to both A2780 and SKOV3 cells. A propidium iodide/annexin V-fluorescein isothiocyanate (V-FITC) double staining assay showed that (-)-asarinin (1) induces apoptotic cell death in ovarian Cancer cells. In addition, (-)-asarinin (1) increased the activation of Caspase-3, Caspase-8, and caspase-9 in ovarian Cancer cells. Pretreatment with Caspase inhibitors attenuated the cell death induced by (-)-asarinin (1). In conclusion, our findings show that (-)-asarinin (1) from the roots of A. sieboldii may induce caspase-dependent apoptotic cell death in human Cancer cells.

Keywords

(−)-asarinin; Asarum sieboldii; apoptosis; caspase; cytotoxicity; ovarian cancer cells.

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