2. Academic Validation
  3. Evolutional conservation of molecular structure and antiviral function of a type I interferon, IFN-kappa, in poultry

Evolutional conservation of molecular structure and antiviral function of a type I interferon, IFN-kappa, in poultry

  • Dev Comp Immunol. 2018 Dec;89:44-53. doi: 10.1016/j.dci.2018.08.004.
Mingchun Gao 1,  Yongli Guo 2,  Jiwen Du 3,  Zhifeng Song 3,  Xiuxin Luo 3,  Junwei Wang 3,  Wenyu Han 4
Affiliations

Affiliations

  • 1 Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Jilin University, Jilin, Changchun, 130062, PR China.
  • 2 Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, 150030, PR China; Animal Disease Prevention and Control Center of Heilongjiang Province, Harbin, Heilongjiang, 150069, PR China.
  • 3 Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, 150030, PR China.
  • 4 Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Jilin University, Jilin, Changchun, 130062, PR China; Jiangsu Coinnovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, Jiangsu, 225009, PR China. Electronic address: hanwy@jlu.edu.cn.
PMID: 30092316 DOI: 10.1016/j.dci.2018.08.004
Abstract

IFN-kappa (IFN-κ) is a type I IFN expressed by keratinocytes, monocytes and dendritic cells with important roles during the innate immune response period. This research was conducted to elaborate the evolution and characteristics of IFN-κ in poultry. Chicken IFN-κ is located on the sex-determining Z chromosome, which is greatly different from mammals. Poultry IFN-κ cluster together in a species-specific manner through positive selection pressure and share only 19-33% homology with mammalian IFN-κ and poultry other type I IFN. Both chicken and duck IFN-κ was constitutively expressed in spleen, skin, lung, and peripheral blood mononuclear cells (PBMC), as well as being significantly induced after treatment with virus in PBMC. Biologically, poultry IFN-κ has Antiviral activity against VSV in chicken embryonic fibroblasts and duck embryonic fibroblasts (CEF and DEF) cells, and induces the expression of IFN stimulated genes (ISGs). After treatment with JAK1 Inhibitor, the ISGs expression can be down-regulated. Overall, our research on poultry IFN-κ not only enriches the knowledge about IFN-κ but also facilitates further research on the role of type I IFNs in Antiviral defense responses in poultry.

Keywords

Antiviral activity; IFN-Kappa; JAK-STAT pathway; Molecular evolutionary; Poultry.

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