1. Academic Validation
  2. Hydrogen Sulfide Promotes Bone Homeostasis by Balancing Inflammatory Cytokine Signaling in CBS-Deficient Mice through an Epigenetic Mechanism

Hydrogen Sulfide Promotes Bone Homeostasis by Balancing Inflammatory Cytokine Signaling in CBS-Deficient Mice through an Epigenetic Mechanism

  • Sci Rep. 2018 Oct 15;8(1):15226. doi: 10.1038/s41598-018-33149-9.
Jyotirmaya Behera 1 Kimberly E Kelly 1 Michael J Voor 2 3 Naira Metreveli 1 Suresh C Tyagi 1 Neetu Tyagi 4
Affiliations

Affiliations

  • 1 Bone Biology Laboratory, Department of Physiology, School of Medicine, University of Louisville, Louisville, KY, 40292, USA.
  • 2 Department of Orthopaedic Surgery, School of Medicine, University of Louisville, Louisville, KY, 40292, USA.
  • 3 Department of Bioengineering, Speed School of Engineering, University of Louisville, Louisville, KY, 40292, USA.
  • 4 Bone Biology Laboratory, Department of Physiology, School of Medicine, University of Louisville, Louisville, KY, 40292, USA. n0tyag01@louisville.edu.
Abstract

Previously, we have shown hyperhomocysteinemia (HHcy) to have a detrimental effect on bone remodeling, which is associated with osteoporosis. During transsulfuration, Hcy is metabolized into hydrogen sulfide (H2S), a gasotransmitter molecule known to regulate bone formation. Therefore, in the present study, we examined whether H2S ameliorates HHcy induced epigenetic and molecular alterations leading to osteoporotic bone loss. To test this mechanism, we employed cystathionine-beta-synthase heterozygote knockout mice, fed with a methionine rich diet (CBS+/- +Met), supplemented with H2S-donor NaHS for 8 weeks. Treatment with NaHS, normalizes plasma H2S, and completely prevents trabecular bone loss in CBS+/- mice. Our data showed that HHcy caused inhibition of HDAC3 activity and subsequent inflammation by imbalancing redox homeostasis. The mechanistic study revealed that inflammatory cytokines (IL-6, TNF-α) are transcriptionally activated by an acetylated lysine residue in histone (H3K27ac) of chromatin by binding to its promoter and subsequently regulating gene expression. A blockade of HDAC3 inhibition in CBS+/- mice by HDAC Activator ITSA-1, led to the remodeling of histone landscapes in the genome and thereby attenuated histone acetylation-dependent inflammatory signaling. We also confirmed that RUNX2 was sulfhydrated by administration of NaHS. Collectively, restoration of H2S may provide a novel treatment for CBS-deficiency induced metabolic osteoporosis.

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Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-100508
    ≥98.0%, HDAC activator