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  3. Mechanical stretch aggravates aortic dissection by regulating MAPK pathway and the expression of MMP-9 and inflammation factors

Mechanical stretch aggravates aortic dissection by regulating MAPK pathway and the expression of MMP-9 and inflammation factors

  • Biomed Pharmacother. 2018 Dec;108:1294-1302. doi: 10.1016/j.biopha.2018.09.129.
Yunnan Hu 1,  Lin Lu 2,  Zhihuang Qiu 3,  Qiuyu Huang 4,  Yinhai Chen 5,  Liangwan Chen 6
Affiliations

Affiliations

  • 1 Department of Cardiac Surgery, Fujian Medical University Union Hospital, Xinquan Road No. 29, Fuzhou, 350001, Fujian, China. Electronic address: huyunnan-105@163.com.
  • 2 Department of Cardiac Surgery, Fujian Medical University Union Hospital, Xinquan Road No. 29, Fuzhou, 350001, Fujian, China. Electronic address: lulinusa@163.com.
  • 3 Department of Cardiac Surgery, Fujian Medical University Union Hospital, Xinquan Road No. 29, Fuzhou, 350001, Fujian, China. Electronic address: qzhflm@126.com.
  • 4 Department of Cardiac Surgery, Fujian Medical University Union Hospital, Xinquan Road No. 29, Fuzhou, 350001, Fujian, China. Electronic address: terryhqy@163.com.
  • 5 Department of Cardiac Surgery, Fujian Medical University Union Hospital, Xinquan Road No. 29, Fuzhou, 350001, Fujian, China. Electronic address: 798956199@qq.com.
  • 6 Department of Cardiac Surgery, Fujian Medical University Union Hospital, Xinquan Road No. 29, Fuzhou, 350001, Fujian, China. Electronic address: chenliangwan@tom.com.
PMID: 30372831 DOI: 10.1016/j.biopha.2018.09.129
Abstract

This study aimed to explore whether mechanical stretch aggravated aortic dissection through regulating MAPK pathway, MMP-9, and inflammation factors. We first established aortic dissection model rats. Mechanical stretch (3 g) was exerted on vascular ring of aortic dissection which was also treated by inhibitors of MAPK pathway (SB203580, SP600125, and U0126). HE and Masson staining showed that aortic dissection severity with 3 g tension was worse than that without tension (0 g); after the treatments of diverse inhibitors, the fracture and breakage of the elastic fibers decreased. The expression of MMP-9, TNF-α, IL-1β) p38/p-p38, JNK1/p-JNK1, and ERK1/2/p-ERK1/2 were determined by immunohistochemical analysis, RT-PCR, and western blot. No matter whether tension was exerted or inhibitors were added, there was no change in the expression of p38, JNK1, and ERK1/2. However, compared to the 0 g group, the expression of MMP-9, TNF-α, IL-1β, p-p38, p-JNK1, and p-ERK1/2 was significantly upregulated in the 3 g group (P < 0.05). In both 0 g and 3 g groups, the expression of MMP-9, TNF-α, IL-1β, p-p38, p-JNK1, and p-ERK1/2 was remarkably downregulated after inhibitors treatment (P < 0.05). In conclusion, mechanical stretch aggravated aortic dissection by regulating the MAPK pathway and the consequent expression of MMP-9 and inflammation factors.

Keywords

Aortic dissection; Inflammation factor; MAPK; MMP-9; Mechanical stretch.

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