2. Academic Validation
  3. M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole

M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole

  • Nat Commun. 2018 Oct 30;9(1):4511. doi: 10.1038/s41467-018-06990-9.
Ning Huang 1,  Donghui Zhang 1,  Fangyuan Li 1,  Peiyuan Chai 1,  Song Wang 1,  Junlin Teng 2,  Jianguo Chen 3 4
Affiliations

Affiliations

  • 1 Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, 100871, Beijing, China.
  • 2 Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, 100871, Beijing, China. junlinteng@pku.edu.cn.
  • 3 Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, 100871, Beijing, China. chenjg@pku.edu.cn.
  • 4 Center for Quantitative Biology, Peking University, 100871, Beijing, China. chenjg@pku.edu.cn.
PMID: 30375385 DOI: 10.1038/s41467-018-06990-9
Abstract

The primary cilium is elongated from the mother centriole and has diverse signaling roles during development and disease. The CP110-CEP97 complex functions as a negative regulator of ciliogenesis, although the mechanisms regulating its mother centriole localization are poorly understood. Here we show that M-Phase Phosphoprotein 9 (MPP9) is recruited by Kinesin Family Member 24 (KIF24) to the distal end of mother centriole where it forms a ring-like structure and recruits CP110-CEP97 by directly binding CEP97. Loss of MPP9 causes abnormal primary cilia formation in growing cells and mouse kidneys. After phosphorylation by Tau Tubulin Kinase 2 (TTBK2) at the beginning of ciliogenesis, MPP9 is targeted for degradation via the ubiquitin-proteasome system, which facilitates the removal of CP110 and CEP97 from the distal end of the mother centriole. Thus, MPP9 acts as a regulator of ciliogenesis by regulating the localization of CP110-CEP97 at the mother centriole.

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