2. Academic Validation
  3. Mechanistic insights into the interactions of NAP1 with the SKICH domains of NDP52 and TAX1BP1

Mechanistic insights into the interactions of NAP1 with the SKICH domains of NDP52 and TAX1BP1

  • Proc Natl Acad Sci U S A. 2018 Dec 11;115(50):E11651-E11660. doi: 10.1073/pnas.1811421115.
Tao Fu 1,  Jianping Liu 1,  Yingli Wang 1,  Xingqiao Xie 1,  Shichen Hu 1,  Lifeng Pan 2
Affiliations

Affiliations

  • 1 State Key Laboratory of Bioorganic and Natural Products Chemistry, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 200032 Shanghai, China.
  • 2 State Key Laboratory of Bioorganic and Natural Products Chemistry, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 200032 Shanghai, China panlf@sioc.ac.cn.
PMID: 30459273 DOI: 10.1073/pnas.1811421115
Abstract

NDP52 and TAX1BP1, two SKIP carboxyl homology (SKICH) domain-containing Autophagy receptors, play crucial roles in selective Autophagy. The autophagic functions of NDP52 and TAX1BP1 are regulated by TANK-binding kinase 1 (TBK1), which may associate with them through the adaptor NAP1. However, the molecular mechanism governing the interactions of NAP1 with NDP52 and TAX1BP1, as well as the effects induced by TBK1-mediated phosphorylation of NDP52 and TAX1BP1, remains elusive. Here, we report the atomic structures of the SKICH regions of NDP52 and TAX1BP1 in complex with NAP1, which not only uncover the mechanistic bases underpinning the specific interactions of NAP1 with the SKICH domains of NDP52 and TAX1BP1 but also reveal the binding mode of a SKICH domain. Moreover, we uncovered that the SKICH domains of NDP52 and TAX1BP1 share a general binding mode to interact with NAP1. Finally, we also evaluated the currently known TBK1-mediated phosphorylation sites in the SKICH domains of NDP52 and TAX1BP1 on the basis of their interactions with NAP1. In all, our findings provide mechanistic insights into the interactions of NAP1 with NDP52 and TAX1BP1, and are valuable for further understanding the functions of these proteins in selective Autophagy.

Keywords

NAP1; NDP52; TAX1BP1; autophagy receptor; selective autophagy.

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