2. Academic Validation
  3. A Small Aromatic Compound Has Antifungal Properties and Potential Anti-Inflammatory Effects against Intestinal Inflammation

A Small Aromatic Compound Has Antifungal Properties and Potential Anti-Inflammatory Effects against Intestinal Inflammation

  • Int J Mol Sci. 2019 Jan 14;20(2):321. doi: 10.3390/ijms20020321.
Clovis Bortolus 1 2 3,  Muriel Billamboz 4 5,  Rogatien Charlet 6 7 8,  Karine Lecointe 9 10 11,  Boualem Sendid 12 13 14,  Alina Ghinet 15 16 17,  Samir Jawhara 18 19 20
Affiliations

Affiliations

  • 1 Institut National de la Santé et de la Recherche Médicale, U995/Team2, F-59000 Lille, France. clovis.bortolus@univ-lille.fr.
  • 2 Lille Inflammation Research International Center, University Lille, U995-LIRIC, F-59000 Lille, France. clovis.bortolus@univ-lille.fr.
  • 3 Service de Parasitologie Mycologie, Pôle de Biologie Pathologie Génétique, CHU Lille, F-59000 Lille, France. clovis.bortolus@univ-lille.fr.
  • 4 Lille Inflammation Research International Center, University Lille, U995-LIRIC, F-59000 Lille, France. muriel.billamboz@yncrea.fr.
  • 5 Laboratoire de Chimie Durable et Santé, Ecole des Hautes Etudes d'Ingénieur (HEI), Yncréa Hauts-de-France, 13 Rue de Toul, F-59046 Lille, France. muriel.billamboz@yncrea.fr.
  • 6 Institut National de la Santé et de la Recherche Médicale, U995/Team2, F-59000 Lille, France. charlet-rogatien@hotmail.fr.
  • 7 Lille Inflammation Research International Center, University Lille, U995-LIRIC, F-59000 Lille, France. charlet-rogatien@hotmail.fr.
  • 8 Service de Parasitologie Mycologie, Pôle de Biologie Pathologie Génétique, CHU Lille, F-59000 Lille, France. charlet-rogatien@hotmail.fr.
  • 9 Institut National de la Santé et de la Recherche Médicale, U995/Team2, F-59000 Lille, France. lecointe.karine@gmail.com.
  • 10 Lille Inflammation Research International Center, University Lille, U995-LIRIC, F-59000 Lille, France. lecointe.karine@gmail.com.
  • 11 Service de Parasitologie Mycologie, Pôle de Biologie Pathologie Génétique, CHU Lille, F-59000 Lille, France. lecointe.karine@gmail.com.
  • 12 Institut National de la Santé et de la Recherche Médicale, U995/Team2, F-59000 Lille, France. boualem.sendid@univ-lille.fr.
  • 13 Lille Inflammation Research International Center, University Lille, U995-LIRIC, F-59000 Lille, France. boualem.sendid@univ-lille.fr.
  • 14 Service de Parasitologie Mycologie, Pôle de Biologie Pathologie Génétique, CHU Lille, F-59000 Lille, France. boualem.sendid@univ-lille.fr.
  • 15 Lille Inflammation Research International Center, University Lille, U995-LIRIC, F-59000 Lille, France. alina.ghinet@yncrea.fr.
  • 16 Laboratoire de Chimie Durable et Santé, Ecole des Hautes Etudes d'Ingénieur (HEI), Yncréa Hauts-de-France, 13 Rue de Toul, F-59046 Lille, France. alina.ghinet@yncrea.fr.
  • 17 Faculty of Chemistry, Al. I. Cuza' University of Iasi, B-dul Carol 1 nr. 11, 700506 Iasi, Romania. alina.ghinet@yncrea.fr.
  • 18 Institut National de la Santé et de la Recherche Médicale, U995/Team2, F-59000 Lille, France. samir.jawhara@inserm.fr.
  • 19 Lille Inflammation Research International Center, University Lille, U995-LIRIC, F-59000 Lille, France. samir.jawhara@inserm.fr.
  • 20 Service de Parasitologie Mycologie, Pôle de Biologie Pathologie Génétique, CHU Lille, F-59000 Lille, France. samir.jawhara@inserm.fr.
PMID: 30646601 DOI: 10.3390/ijms20020321
Abstract

Resistance of the opportunistic pathogen Candida albicans to Antifungal drugs has increased significantly in recent years. After screening 55 potential Antifungal compounds from a chemical library, 2,3-dihydroxy-4-methoxybenzaldehyde (DHMB) was identified as having potential Antifungal activity. The properties of DHMB were then assessed in vitro and in vivo against C. albicans overgrowth and intestinal inflammation. Substitution on the aromatic ring of DHMB led to a strong decrease in its biological activity against C. albicans. The MIC of DHMB was highly effective at eliminating C. albicans when compared to that of caspofungin or fluconazole. Additionally, DHMB was also effective against clinically isolated fluconazole- or caspofungin-resistant C. albicans strains. DHMB was administered to Animals at high doses. This compound was not cytotoxic and was well-tolerated. In experimental dextran sodium sulphate (DSS)-induced colitis in mice, DHMB reduced the clinical and histological score of inflammation and promoted the elimination of C. albicans from the gut. This finding was supported by a decrease in aerobic bacteria while anaerobic bacteria populations were re-established in mice treated with DHMB. DHMB is a small organic molecule with Antifungal properties and anti-inflammatory activity by exerting protective effects on intestinal epithelial cells.

Keywords

2,3-dihydroxy-4-methoxyBenzaldehyde; Candida albicans; aerobic bacteria; anaerobic bacteria; colitis; melanin.

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