2. Academic Validation
  3. Isolation, structural elucidation, and insights into the anti-inflammatory effects of triterpene saponins from the leaves of Stauntonia hexaphylla

Isolation, structural elucidation, and insights into the anti-inflammatory effects of triterpene saponins from the leaves of Stauntonia hexaphylla

  • Bioorg Med Chem Lett. 2019 Apr 15;29(8):965-969. doi: 10.1016/j.bmcl.2019.02.022.
Le Ba Vinh 1,  Hyun-Jae Jang 2,  Nguyen Viet Phong 3,  Kyoungwon Cho 4,  Sung Sun Park 4,  Jong Seong Kang 5,  Young Ho Kim 6,  Seo Young Yang 7
Affiliations

Affiliations

  • 1 College of Pharmacy, Chungnam National University, Daejeon 34134, Republic of Korea; Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology, Hanoi, Viet Nam.
  • 2 Immunoregulatory Material Research Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeonbuk 56212, Republic of Korea.
  • 3 Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology, Hanoi, Viet Nam.
  • 4 Chong Kun Dang Healthcare Corporation, 47, Beodeunaru-ro, Yeongdeungpo-gu, Seoul 07249, Republic of Korea.
  • 5 College of Pharmacy, Chungnam National University, Daejeon 34134, Republic of Korea.
  • 6 College of Pharmacy, Chungnam National University, Daejeon 34134, Republic of Korea. Electronic address: yhk@cnu.ac.kr.
  • 7 College of Pharmacy, Chungnam National University, Daejeon 34134, Republic of Korea. Electronic address: syyang@cnu.ac.kr.
PMID: 30808589 DOI: 10.1016/j.bmcl.2019.02.022
Abstract

Using various chromatographic techniques, 23 triterpene saponins (1-23) were isolated from an ethanol extract of Stauntonia hexaphylla, including two new compounds (12 and 15). Their chemical structures were established by comprehensive spectroscopic methods such as 1D- and 2D-NMR, and HR-ESI-MS, and chemical reactions. The anti-inflammatory activities of the isolated saponins were determined using the nitric oxide (NO) assay. Compound 13 exhibited the greatest inhibitory effect (IC50 = 0.59 μM). In addition to NO, compound 13 suppressed the secretion of PGE2, IL-1β, and IL-6, but not TNF-α, and inhibited the protein expression of iNOS and COX-2 in LPS-activated RAW264.7 cells. The chemical derivatives of the isolated compounds were studied using structure-activity relationships. The results suggested that compound 13 isolated from S. hexaphylla might be useful for treating inflammation. This is the first comprehensive study of saponins from the leaves of S. hexaphylla based on anti-inflammatory extract screening guidelines.

Keywords

Anti-inflammatory effect; NO; Stauntonia hexaphylla; Stauntonoside A; Stauntonoside B; Triterpenoid saponins.

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