2. Academic Validation
  3. Phenolic Compounds from Humulus lupulus as Natural Antimicrobial Products: New Weapons in the Fight against Methicillin Resistant Staphylococcus aureus, Leishmania mexicana and Trypanosoma brucei Strains

Phenolic Compounds from Humulus lupulus as Natural Antimicrobial Products: New Weapons in the Fight against Methicillin Resistant Staphylococcus aureus, Leishmania mexicana and Trypanosoma brucei Strains

  • Molecules. 2019 Mar 14;24(6):1024. doi: 10.3390/molecules24061024.
Laetitia Bocquet 1,  Sevser Sahpaz 2,  Natacha Bonneau 3,  Claire Beaufay 4,  Séverine Mahieux 5,  Jennifer Samaillie 6,  Vincent Roumy 7,  Justine Jacquin 8,  Simon Bordage 9,  Thierry Hennebelle 10,  Feng Chai 11,  Joëlle Quetin-Leclercq 12,  Christel Neut 13,  Céline Rivière 14
Affiliations

Affiliations

  • 1 EA 7394-ICV, Charles Viollette Research Institute, SFR Condorcet FR CNRS 3417, Univ. Lille, INRA, ISA-Yncréa, Univ. Artois, University Littoral Côte d'Opale, 3 rue du Professeur Laguesse, 59000 Lille, France. laetitia.bocquet1309@gmail.com.
  • 2 EA 7394-ICV, Charles Viollette Research Institute, SFR Condorcet FR CNRS 3417, Univ. Lille, INRA, ISA-Yncréa, Univ. Artois, University Littoral Côte d'Opale, 3 rue du Professeur Laguesse, 59000 Lille, France. sevser.sahpaz@univ-lille.fr.
  • 3 EA 7394-ICV, Charles Viollette Research Institute, SFR Condorcet FR CNRS 3417, Univ. Lille, INRA, ISA-Yncréa, Univ. Artois, University Littoral Côte d'Opale, 3 rue du Professeur Laguesse, 59000 Lille, France. natacha.bonneau@univ-lille.fr.
  • 4 Pharmacognosy Research group, Louvain Drug Research Institute, Université Catholique de Louvain, 1200 Brussels, Belgium. claire.beaufay@uclouvain.be.
  • 5 U995-LIRIC, Lille Inflammation Research International Center, University Lille, Inserm, CHU Lille, 59000 Lille, France. severine.mahieux@univ-lille.fr.
  • 6 EA 7394-ICV, Charles Viollette Research Institute, SFR Condorcet FR CNRS 3417, Univ. Lille, INRA, ISA-Yncréa, Univ. Artois, University Littoral Côte d'Opale, 3 rue du Professeur Laguesse, 59000 Lille, France. jennifer.samaillie@univ-lille.fr.
  • 7 EA 7394-ICV, Charles Viollette Research Institute, SFR Condorcet FR CNRS 3417, Univ. Lille, INRA, ISA-Yncréa, Univ. Artois, University Littoral Côte d'Opale, 3 rue du Professeur Laguesse, 59000 Lille, France. vincent.roumy@univ-lille.fr.
  • 8 EA 7394-ICV, Charles Viollette Research Institute, SFR Condorcet FR CNRS 3417, Univ. Lille, INRA, ISA-Yncréa, Univ. Artois, University Littoral Côte d'Opale, 3 rue du Professeur Laguesse, 59000 Lille, France. justine.jacquin@yncrea.fr.
  • 9 EA 7394-ICV, Charles Viollette Research Institute, SFR Condorcet FR CNRS 3417, Univ. Lille, INRA, ISA-Yncréa, Univ. Artois, University Littoral Côte d'Opale, 3 rue du Professeur Laguesse, 59000 Lille, France. simon.bordage@univ-lille.fr.
  • 10 EA 7394-ICV, Charles Viollette Research Institute, SFR Condorcet FR CNRS 3417, Univ. Lille, INRA, ISA-Yncréa, Univ. Artois, University Littoral Côte d'Opale, 3 rue du Professeur Laguesse, 59000 Lille, France. thierry.hennebelle@univ-lille.fr.
  • 11 U1008-Controlled Drug Delivery Systems and Biomaterials, University Lille, Inserm, CHU Lille, 59000 Lille, France. feng.chai@univ-lille.fr.
  • 12 Pharmacognosy Research group, Louvain Drug Research Institute, Université Catholique de Louvain, 1200 Brussels, Belgium. joelle.leclercq@uclouvain.be.
  • 13 U995-LIRIC, Lille Inflammation Research International Center, University Lille, Inserm, CHU Lille, 59000 Lille, France. christel.neut@univ-lille.fr.
  • 14 EA 7394-ICV, Charles Viollette Research Institute, SFR Condorcet FR CNRS 3417, Univ. Lille, INRA, ISA-Yncréa, Univ. Artois, University Littoral Côte d'Opale, 3 rue du Professeur Laguesse, 59000 Lille, France. celine.riviere@univ-lille.fr.
PMID: 30875854 DOI: 10.3390/molecules24061024
Abstract

New anti-infective agents are urgently needed to fight microbial resistance. Methicillin-resistant Staphylococcus aureus (MRSA) strains are particularly responsible for complicated pathologies that are difficult to treat due to their virulence and the formation of persistent biofilms forming a complex protecting shell. Parasitic infections caused by Trypanosoma brucei and Leishmania mexicana are also of global concern, because of the mortality due to the low number of safe and effective treatments. Female inflorescences of hop produce specialized metabolites known for their antimicrobial effects but underexploited to fight against drug-resistant Microorganisms. In this study, we assessed the antimicrobial potential of phenolic compounds against MRSA clinical isolates, T. brucei and L. mexicana. By fractionation process, we purified the major prenylated Chalcones and acylphloroglucinols, which were quantified by UHPLC-UV in different plant parts, showing their higher content in the active flowers extract. Their potent Antibacterial action (MIC < 1 µg/mL for the most active compound) was demonstrated against MRSA strains, through kill curves, post-antibiotic effects, anti-biofilm assays and synergy studies with Antibiotics. An antiparasitic activity was also shown for some purified compounds, particularly on T. brucei (IC50 < 1 to 11 µg/mL). Their cytotoxic activity was assessed both on Cancer and non-cancer human cell lines.

Keywords

Humulus lupulus; Leishmania mexicana mexicana; Trypanosoma brucei brucei; antimicrobial agents; methicillin-resistant Staphylococcus aureus; prenylated phenolic compounds.

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