2. Academic Validation
  3. Reflexin A, a new indole alkaloid from Rauvolfia reflexa induces apoptosis against colon cancer cells

Reflexin A, a new indole alkaloid from Rauvolfia reflexa induces apoptosis against colon cancer cells

  • J Asian Nat Prod Res. 2020 May;22(5):474-488. doi: 10.1080/10286020.2019.1588888.
Mehran Fadaeinasab 1,  Hamed Karimian 2,  Hanita Omar 3,  Hairin Taha 4,  Arash Khorasani 5,  Behrooz Banisalam 5,  Kamal Aziz Ketuly 6,  Zanariah Abdullah 1
Affiliations

Affiliations

  • 1 Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia.
  • 2 School of Medicine, Taylor's University, Subang Jaya Selangor 47500, Malaysia.
  • 3 Centre for Foundation Studies in Science, Division of Chemistry, University of Malaya, Kuala Lumpur 50603, Malaysia.
  • 4 Institute of Energy Infrastructure, Universiti Tenaga Nasional, Kajang 43000, Malaysia.
  • 5 Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia.
  • 6 Department of Medical Chemistry, College of Medicine, University of Duhok, Duhok 78, Kurdistan Region, Iraq.
PMID: 30945944 DOI: 10.1080/10286020.2019.1588888
Abstract

One new indole alkaloid, reflexin A (1), and two known indoles, macusine B (2) and vinorine (3), were isolated from the bark of Rauvolfia reflexa. Their structures were elucidated by 1D and 2D NMR, UV, IR, and MS spectroscopic analyses. Compound 1 displayed Anticancer activity against HCT-116 colon Cancer cells with an IC50 value of 30.24 ± 0.75 µM. The results implied that the newly isolated 1 induced Apoptosis in HCT-116 cells, suggesting its possible role as an Anticancer agent. In vivo acute toxicity study was performed on compound 1 to evaluate its safety profile.

Keywords

HCT-116 cells; Rauvolfia reflexa; acute toxicity; apoptosis; indole alkaloids; reflexin A.

Figures
Products