2. Academic Validation
  3. PATZ1 is required for efficient HIV-1 infection

PATZ1 is required for efficient HIV-1 infection

  • Biochem Biophys Res Commun. 2019 Jun 25;514(2):538-544. doi: 10.1016/j.bbrc.2019.04.175.
Ishmael Dzigbordi Aziati 1,  Takeshi Yoshida 2,  Akiko Hamano 1,  Kenjiro Maeda 1,  Hiroaki Takeuchi 1,  Shoji Yamaoka 3
Affiliations

Affiliations

  • 1 Department of Molecular Virology, Tokyo Medical and Dental University (TMDU), Tokyo, Japan.
  • 2 Department of Molecular Virology, Tokyo Medical and Dental University (TMDU), Tokyo, Japan. Electronic address: takeshi-yoshida@umin.ac.jp.
  • 3 Department of Molecular Virology, Tokyo Medical and Dental University (TMDU), Tokyo, Japan. Electronic address: shojmmb@tmd.ac.jp.
PMID: 31060775 DOI: 10.1016/j.bbrc.2019.04.175
Abstract

Successful HIV-1 Infection and subsequent replication deeply depend on how the virus usurps the host cell machinery. Identification and functional characterization of these host factors may represent a critical strategy for developing novel anti-HIV-1 therapy. Here, expression cloning with a cDNA expression library identified as an inhibitor of HIV-1 Infection, a carboxy-terminally truncated form of human POZ/BTB and AT-hook- containing Zinc finger protein 1 (PATZ1), a transcriptional regulatory factor implicated in development and Cancer. Knockdown or knockout of endogenous PATZ1 revealed a supportive role of PATZ1 in HIV-1 Infection, but not in transduction with murine leukemia virus-based retroviral vector. More specifically, knockdown or knockout of PATZ1 impaired the viral cDNA synthesis but not the entry process and expression of two PATZ1 isoforms in PATZ1-KO cells restored susceptibility to HIV-1 Infection. These results indicate that PATZ1 plays an important role in HIV-1 Infection.

Keywords

HIV-1; Host factor; Infection; PATZ1; Reverse transcription; Virus.

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