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  3. Visualization of viral DNA assembly using nonfluorescent staining

Visualization of viral DNA assembly using nonfluorescent staining

  • Biotech Histochem. 2019 Jul;94(5):309-312. doi: 10.1080/10520295.2018.1530453.
E M Karalova 1,  L H Hakobyan 1,  M R Tatoyan 2,  K T Sahakyan 2,  Z A Karalyan 1 2 3
Affiliations

Affiliations

  • 1 a Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS , Yerevan , Armenia.
  • 2 b Department of Histology, Cytology and Embryology, Yerevan State Medical University , Yerevan , Armenia.
  • 3 c Department of Bioengineering, Bioinformatics and Molecular Biology, Russian-Armenian (Slavonic) University , Yerevan , Armenia.
PMID: 31066292 DOI: 10.1080/10520295.2018.1530453
Abstract

We present an easy test for rapid visualization of viral DNA assemblies in infected cell cytoplasm. We selected the best stains for nuclear staining: Nile blue A, Bismarck brown, gallocyanin chrome alum, methyl green pyronin and azure II. None of the staining techniques is fluorescent, which facilitates their use in everyday experiments. Methyl green is most promising for routine detection of viral DNA assemblies in the cytoplasm; the procedure enables ready detection of viral DNA accumulation in the cytoplasm.

Keywords

DNA; methyl green pyronin; nonfluorescent staining; viral assemblies.

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