2. Academic Validation
  3. A new lateral root growth inhibitor from the sponge-derived fungus Aspergillus sp. LS45

A new lateral root growth inhibitor from the sponge-derived fungus Aspergillus sp. LS45

  • Bioorg Med Chem Lett. 2019 Jul 1;29(13):1593-1596. doi: 10.1016/j.bmcl.2019.04.051.
Liming Huang 1,  Lijian Ding 2,  Xiaohui Li 1,  Ning Wang 1,  Yushan Yan 3,  Mengxiang Yang 3,  Wei Cui 3,  C Benjamin Naman 1,  Kejun Cheng 4,  Weiyan Zhang 1,  Bin Zhang 1,  Haixiao Jin 1,  Shan He 5
Affiliations

Affiliations

  • 1 Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, 315800 Ningbo, PR China.
  • 2 Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, 315800 Ningbo, PR China. Electronic address: dinglijian@nbu.edu.cn.
  • 3 School of Medicine, Ningbo University, 315211 Ningbo, PR China.
  • 4 College of Ecology, Lishui University, 323000 Lishui, PR China.
  • 5 Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, 315800 Ningbo, PR China. Electronic address: heshan@nbu.edu.cn.
PMID: 31078410 DOI: 10.1016/j.bmcl.2019.04.051
Abstract

Two new γ-lactones, aspergilactones A (1) and B (2), were discovered along with two known compounds, annularin A (3) and pericoterpenoid A (4), from a culture of the sponge-associated fungus Aspergillus sp. LS45. The planar structures of 1-4 were characterized using comprehensive spectroscopic methods and comparison with literature data. The absolute configurations of 1 and 2 were determined by comparison of electronic circular dichroism (ECD) spectroscopic and optical rotation data with those of known analogues as well as calculated ECD analysis. Compounds 1-4 were tested in a variety of bioassays, and both 1 and 4 exhibited significant inhibition against the lateral root growth of Arabidopsis thaliana Columbia-0 at a concentration of 100 μM. In addition, the in vitro cytotoxic activities of 1-4 against six human Cancer cell lines CCRF-CEM, K562, BGC823, AGS, HCT-116 and MDA-MB-231 were evaluated. Compound 4 showed moderate inhibitory effects on CCRF-CEM and K562 Cancer cell lines with IC50 values of 13.8 ± 1.6 and 12.9 ± 2.5 μM, respectively. However, compounds 1-4 did not show any notable AChE inhibitory activity in vitro.

Keywords

AChE; Aspergillus sp.; Cytotoxicity; Lateral root growth; Sponge-derived fungus; γ-Lactone.

Figures