2. Academic Validation
  3. Core-Fucosylated Tetra-Antennary N-Glycan Containing A Single N-Acetyllactosamine Branch Is Associated with Poor Survival Outcome in Breast Cancer

Core-Fucosylated Tetra-Antennary N-Glycan Containing A Single N-Acetyllactosamine Branch Is Associated with Poor Survival Outcome in Breast Cancer

  • Int J Mol Sci. 2019 May 23;20(10):2528. doi: 10.3390/ijms20102528.
Harmin Herrera 1,  Tinslee Dilday 2,  Allison Uber 3,  Danielle Scott 4,  Joelle N Zambrano 5,  Mengjun Wang 6,  Peggi M Angel 7,  Anand S Mehta 8,  Richard R Drake 9,  Elizabeth G Hill 10,  Elizabeth S Yeh 11
Affiliations

Affiliations

  • 1 Graduate School of Biomedical Sciences and Professional Studies, Microbiology and Immunology Graduate Program, Drexel University College of Medicine, Philadelphia, PA 19129, USA. hh435@drexel.edu.
  • 2 Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, SC 29425, USA. dilday@musc.edu.
  • 3 Department of Pediatrics, Division of Hematology/Oncology, Medical University of South Carolina, Charleston, SC 29425, USA. uber@musc.edu.
  • 4 Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, SC 29425, USA. scottd@musc.edu.
  • 5 Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, SC 29425, USA. zambrano@musc.edu.
  • 6 Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, SC 29425, USA. wangm@musc.edu.
  • 7 Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, SC 29425, USA. angelp@musc.edu.
  • 8 Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, SC 29425, USA. mehtaa@musc.edu.
  • 9 Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, SC 29425, USA. draker@musc.edu.
  • 10 Department of Public Health Sciences, Medical University of South Carolina, Charleston, SC 29425, USA. hille@musc.edu.
  • 11 Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, SC 29425, USA. esyeh@iu.edu.
PMID: 31126011 DOI: 10.3390/ijms20102528
Abstract

(1) Glycoproteins account for ~80% of proteins located at the cell surface and in the extracellular matrix. A growing body of evidence indicates that α-L-fucose protein modifications contribute to breast Cancer progression and metastatic disease. (2) Using a combination of techniques, including matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) based in cell and on tissue imaging and glycan Sequencing using exoglycosidase analysis coupled to hydrophilic interaction ultra-high performance liquid chromatography (HILIC UPLC), we establish that a core-fucosylated tetra-antennary glycan containing a single N-acetyllactosamine (F(6)A4G4Lac1) is associated with poor clinical outcomes in breast Cancer, including lymph node metastasis, recurrent disease, and reduced survival. (3) This study is the first to identify a single N-glycan, F(6)A4G4Lac1, as having a correlation with poor clinical outcomes in breast Cancer.

Keywords

N-acetyllactosamine; N-glycan; breast cancer; core-fucose.

Figures
Products