2. Academic Validation
  3. Feasibility of repairing full-thickness skin defects by iPSC-derived epithelial stem cells seeded on a human acellular amniotic membrane

Feasibility of repairing full-thickness skin defects by iPSC-derived epithelial stem cells seeded on a human acellular amniotic membrane

  • Stem Cell Res Ther. 2019 May 31;10(1):155. doi: 10.1186/s13287-019-1234-9.
Huateng Zhou 1,  Lixiang Wang 2,  Cui Zhang 3,  Jintao Hu 1,  Jianlin Chen 3,  Weibin Du 2,  Fei Liu 4,  Weifan Ren 1,  Jinfu Wang 5,  Renfu Quan 6
Affiliations

Affiliations

  • 1 Clinical Medical College, Zhejiang Chinese Medical University, Zhejiang, 310053, Hangzhou, China.
  • 2 Department of Orthopedic Surgery, Xiaoshan Traditional Chinese Medical Hospital, Zhejiang, 311200, Hangzhou, China.
  • 3 Laboratory of Stem Cells, Institute of Cell Biology, College of Life Sciences, Zhejiang University, Zhejiang, 310058, Hangzhou, China.
  • 4 Department of Chinese Medicine Rehabilitation, Xiushan People's Hospital, Xiushan, Chongqing, 409900, China.
  • 5 Laboratory of Stem Cells, Institute of Cell Biology, College of Life Sciences, Zhejiang University, Zhejiang, 310058, Hangzhou, China. wjfu@zju.edu.cn.
  • 6 Clinical Medical College, Zhejiang Chinese Medical University, Zhejiang, 310053, Hangzhou, China. quanrenfu@126.com.
PMID: 31151466 DOI: 10.1186/s13287-019-1234-9
Abstract

Background: Induced pluripotent stem cells (iPSCs) can generate epithelial stem cells (EpSCs) as seed cells for skin substitutes to repair skin defects. Here, we investigated the effects of a human acellular amniotic membrane (hAAM) combined with iPSC-derived CD200+/ITGA6+ EpSCs as a skin substitute on repairing skin defects in nude mice.

Methods: Human urinary cells isolated from a healthy donor were reprogrammed into iPSCs and then induced into CD200+/ITGA6+ epithelial stem cells. Immunocytochemistry and RT-PCR were used to examine the characteristics of the induced epithelial stem cells. iPSC-derived EpSCs were cultured on a hAAM, and cytocompatibility of the composite was analyzed by CCK8 assays and scanning electron microscopy. Then, hAAMs combined with iPSC-derived EpSCs were transplanted onto skin defects of mice. The effects of this composite on skin repair were evaluated by immunohistochemistry.

Results: The results showed that CD200+/ITGA6+ epithelial stem cells induced from iPSCs displayed the phenotypes of hair follicle stem cells. After seeding on the hAAM, iPSC-derived epithelial stem cells had the ability to proliferate. After transplantation, CD200+/ITGA6+ epithelial stem cells on the hAAM promoted the construction of hair follicles and interfollicular epidermis.

Conclusions: These results indicated that transplantation of a hAAM combined with iPS-derived EpSCs is feasible to reconstruct skin and skin appendages, and may be a substantial reference for iPSC-based therapy for skin defects.

Keywords

CD200+/ITGA6+ epithelial stem cells; Hair follicle; Induced pluripotent stem cells; Skin defect.

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