2. Academic Validation
  3. Akt plays indispensable roles during the first cell lineage differentiation of mouse

Akt plays indispensable roles during the first cell lineage differentiation of mouse

  • J Mol Histol. 2019 Aug;50(4):369-374. doi: 10.1007/s10735-019-09833-z.
Songhua Xu 1 2,  Lili Pang 1,  Yue Liu 1,  Xiuli Lian 2,  Kaien Mo 2,  Ruimin Lv 2,  Huimin Zhu 3 4,  Chengyu Lv 2,  Jianmin Lin 2,  Jiandong Sun 2,  Lixuan Xu 2,  Shie Wang 5 6
Affiliations

Affiliations

  • 1 Key Laboratory of Stem Cell Engineering and Regenerative Medicine of Fujian Province University, Fuzhou, 350122, People's Republic of China.
  • 2 Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, People's Republic of China.
  • 3 Fujian Key Laboratory of Medical Bioinformatics, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, People's Republic of China.
  • 4 Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, People's Republic of China.
  • 5 Key Laboratory of Stem Cell Engineering and Regenerative Medicine of Fujian Province University, Fuzhou, 350122, People's Republic of China. shiewang@fjmu.edu.cn.
  • 6 Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, People's Republic of China. shiewang@fjmu.edu.cn.
PMID: 31190160 DOI: 10.1007/s10735-019-09833-z
Abstract

The first cell lineage differentiation occurs during the development of mouse 8-cell embryo to blastocyst. Akt is a potent kinase whose role during blastocyst formation has not been elucidated. In the present study, immunofluorescence results showed that the Akt protein was specifically localized to the outer cells of the morula. Akt-specific inhibitor MK2206 significantly inhibited mouse blastocyst formation and resulted in decreased expression of the trophectoderm marker Cdx2 and led to granular distribution of ERα in the cytoplasm. Furthermore, knockdown of ERα by siRNA microinjection can also lead to a decrease in the development rate of mouse blastocysts, accompanied by a decrease in the expression level of YAP protein. We conclude that Akt may be indispensable for the first cell lineage differentiation of mouse.

Keywords

Akt; Blastocyst formation; Immunofluorescence; Mouse; Yap.

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