2. Academic Validation
  3. Brominated Azaphilones from the Sponge-Associated Fungus Penicillium canescens Strain 4.14.6a

Brominated Azaphilones from the Sponge-Associated Fungus Penicillium canescens Strain 4.14.6a

  • J Nat Prod. 2019 Aug 23;82(8):2159-2166. doi: 10.1021/acs.jnatprod.9b00151.
Marian Frank 1,  Rudolf Hartmann 2,  Malte Plenker 2,  Attila Mándi 3,  Tibor Kurtán 3,  Ferhat Can Özkaya 4,  Werner E G Müller 5,  Matthias U Kassack 6,  Alexandra Hamacher 6,  Wenhan Lin 7,  Zhen Liu 1,  Peter Proksch 1
Affiliations

Affiliations

  • 1 Institute of Pharmaceutical Biology and Biotechnology , Heinrich-Heine-Universität Düsseldorf , Universitätsstrasse 1 , 40225 Düsseldorf , Germany.
  • 2 Institute of Complex Systems: Strukturbiochemie , Forschungszentrum Juelich , Wilhelm-Johnen-Strasse , 52428 Juelich , Germany.
  • 3 Department of Organic Chemistry , University of Debrecen , Egyetem tér 1 , Debrecen 4032 , Hungary.
  • 4 Faculty of Fisheries , İzmir Katip Çelebi University , Çiğli, 35620 İzmir , Turkey.
  • 5 Institute of Physiological Chemistry , Universitätsmedizin der Johannes Gutenberg-Universität Mainz , 55128 Mainz , Germany.
  • 6 Institute for Pharmaceutical and Medicinal Chemistry , Heinrich-Heine-Universität Düsseldorf , Universitätsstrasse 1 , 40225 Düsseldorf , Germany.
  • 7 State Key Laboratory of Natural and Biomimetic Drugs , Peking University , Beijing 100191 , China.
PMID: 31359750 DOI: 10.1021/acs.jnatprod.9b00151
Abstract

The fungus Penicillium canescens was isolated from the inner tissue of the Mediterranian Sponge Agelas oroides. Fermentation of the fungus on solid rice medium yielded one new chlorinated diphenyl ether (1) and 13 known compounds (2-14). Addition of 5% NaBr to the rice medium increased the amounts of 4-6, while lowering the amounts of 8, 12, and 14. Furthermore, it induced the accumulation of 17 and two new brominated azaphilones, bromophilones A and B (15 and 16). Compounds 15 and 16 are the first example of azaphilones with the connection of a benzene moiety and the pyranoquinone core through a methylene group. The structures of the new compounds were elucidated based on the 1D and 2D NMR spectra as well as on HRESIMS data. The absolute configuration of the condensed bicyclic moiety of 15 and 16 was determined by sTDA ECD calculations. Compound 16 exhibited moderate cytotoxicity against the mouse lymphoma cell line L5178Y (IC50 8.9 μM), as well as against the human ovarian Cancer cell line A2780 (IC50 2.7 μM), whereas the stereoisomer 15 was considerably less active.

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