2. Academic Validation
  3. Ac2-26 ameliorates lung ischemia-reperfusion injury via the eNOS pathway

Ac2-26 ameliorates lung ischemia-reperfusion injury via the eNOS pathway

  • Biomed Pharmacother. 2019 Sep:117:109194. doi: 10.1016/j.biopha.2019.109194.
Jing Gong 1,  Ying-Nan Ju 2,  Xue-Ting Wang 3,  Jing-Li Zhu 4,  Zhe-Hao Jin 5,  Wei Gao 6
Affiliations

Affiliations

  • 1 Anesthesiology Department, The Second Affiliated Hospital of Harbin Medical University, 246 Xuefu Road, Nangang District, Harbin 150000, China. Electronic address: justsqueirrel@126.com.
  • 2 Department of ICU, The Tumor Hospital of Harbin Medical University, 150 Haping Road, Harbin 150081, China. Electronic address: juyingnan2010@126.com.
  • 3 Anesthesiology Department, The Second Affiliated Hospital of Harbin Medical University, 246 Xuefu Road, Nangang District, Harbin 150000, China. Electronic address: 793461764@qq.com.
  • 4 Anesthesiology Department, The Second Affiliated Hospital of Harbin Medical University, 246 Xuefu Road, Nangang District, Harbin 150000, China. Electronic address: 1210268654@qq.com.
  • 5 Anesthesiology Department, The Second Affiliated Hospital of Harbin Medical University, 246 Xuefu Road, Nangang District, Harbin 150000, China. Electronic address: zhehao120@163.com.
  • 6 Anesthesiology Department, The Second Affiliated Hospital of Harbin Medical University, 246 Xuefu Road, Nangang District, Harbin 150000, China. Electronic address: gaowei20055@126.com.
PMID: 31387174 DOI: 10.1016/j.biopha.2019.109194
Abstract

Background: Lung ischemia-reperfusion injury (LIRI) is a major complication after lung transplantation. Annexin A1 (AnxA1) ameliorates inflammation in various injured organs. This study aimed to determine the effects and mechanism of AnxA1 on LIRI after lung transplantation.

Methods: Thirty-two rats were randomized into sham, saline, Ac2-26 and Ac2-26/L groups. Rats in the saline, Ac2-26 and Ac2-26/L groups underwent left lung transplantation and received saline, Ac2-26, and Ac2-26/L-NIO, respectively. After 24 h of reperfusion, serum and transplanted lung tissues were examined.

Results: The partial pressure of oxygen (PaO2) was increased in the Ac2-26 group compared to that in the saline group but was decreased by L-NIO treatment. In the Ac2-26 group, the wet-to-dry (W/D) weight ratios, total protein concentrations, proinflammatory factors and inducible nitric oxide synthase levels were notably decreased, but the concentrations of anti-inflammatory factors and endothelial nitric oxide synthase levels were significantly increased. Ac2-26 attenuated histological injury and cell Apoptosis, and this improvement was reversed by L-NIO.

Conclusions: Ac2-26 reduced LIRI and improved alveoli-capillary permeability by inhibiting oxygen stress, inflammation and Apoptosis. The protective effect of Ac2-26 on LIRI largely depended on the endothelial nitric oxide synthase pathway.

Keywords

Ac2-26; Annexin A1; Ischemia/reperfusion injury; Lung; Transplantation.

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