2. Academic Validation
  3. Natural neuroprotective alkaloids from Stephania japonica (Thunb.) Miers

Natural neuroprotective alkaloids from Stephania japonica (Thunb.) Miers

  • Bioorg Chem. 2019 Oct:91:103175. doi: 10.1016/j.bioorg.2019.103175.
Jiao Xiao 1,  Tingyu Hao 2,  Gang Chen 1,  Junyu Song 1,  Bin Lin 3,  Wei Li 4,  Jikai Xu 2,  Jingyu Liu 2,  Yue Hou 5,  Ning Li 6
Affiliations

Affiliations

  • 1 School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, PR China.
  • 2 College of Life and Health Sciences, Northeastern University, Shenyang 110004, PR China; State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin, PR China.
  • 3 School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, PR China.
  • 4 School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, PR China; Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, Japan.
  • 5 College of Life and Health Sciences, Northeastern University, Shenyang 110004, PR China; State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin, PR China. Electronic address: houyue@mail.neu.edu.cn.
  • 6 School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, PR China; State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin, PR China. Electronic address: liningsypharm@163.com.
PMID: 31398598 DOI: 10.1016/j.bioorg.2019.103175
Abstract

Modulating inflammatory responses after stroke can prevent brain injury and, therefore, improve neurological outcome. Stephania japonica (Thunb.) Miers is a Chinese folk medicine with the function of dispelling the "wind and blockage" in the human body according to the Chinese medicine theory, in which the symptoms of stroke are caused by the "wind and blockage" in the body. In this paper, we for the first time linked S. japonica to stroke by clarifying fifteen alkaloidal constituents including five undescribed (1-5) ones and screening out six hasubanan type Alkaloids (1-4, 7, 15) that elicited stronger anti-neuroinflammatory activities than the positive drug. Moreover, the total alkaloid fraction (ASJ) with previously undescribed 3 as the main component was subject to the in vivo evaluation of the protective effect in the MCAO-induced brain injury. The results showed that ASJ exhibited potent protective effect against brain injury in the MCAO rat model. The results reported in this paper suggested that the hasubanan Alkaloids from S. japonica would be an important molecular source for discovering novel therapeutic agents for neuroinflammation-related diseases, such as stroke diseases.

Keywords

Hasubanan alkaloids; Neuroinflammation; Stephania japonica; Stroke.

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