2. Academic Validation
  3. Antioxidant and Cytoprotective Effects of (-)-Epigallocatechin-3-(3″- O-methyl) Gallate

Antioxidant and Cytoprotective Effects of (-)-Epigallocatechin-3-(3″- O-methyl) Gallate

  • Int J Mol Sci. 2019 Aug 16;20(16):3993. doi: 10.3390/ijms20163993.
Eunji Kim 1,  Sang Yun Han 1 2,  Kyeonghwan Hwang 3,  Donghyun Kim 3,  Eun-Mi Kim 3,  Mohammad Amjad Hossain 4,  Jong-Hoon Kim 5,  Jae Youl Cho 6
Affiliations

Affiliations

  • 1 Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Korea.
  • 2 Daewoong Pharmaceutical Co., Yongin 17028, Korea.
  • 3 Basic Research & Innovation vision, R&D Center, AmorePacific Corporation, Yongin 17074, Korea.
  • 4 College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Korea.
  • 5 College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Korea. jhkim1@jbnu.ac.kr.
  • 6 Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Korea. jaecho@skku.edu.
PMID: 31426336 DOI: 10.3390/ijms20163993
Abstract

Reactive Oxygen Species (ROS) are generated from diverse cellular processes or external sources such as chemicals, pollutants, or ultraviolet (UV) irradiation. Accumulation of radicals causes cell damage that can result in degenerative diseases. Antioxidants remove radicals by eliminating unpaired electrons from other molecules. In skin health, antioxidants are essential to protect cells from the environment and prevent skin aging. (-)-Epigallocatechin-3-(3″-O-methyl) gallate (3″Me-EGCG) has been found in limited oolong teas or green teas with distinctive methylated form, but its precise activities have not been fully elucidated. In this study, we examined the antioxidant roles of 3″Me-EGCG in keratinocytes (HaCaT cells). 3″Me-EGCG showed scavenging effects in cell and cell-free systems. Under H2O2 exposure, 3″Me-EGCG recovered cell viability and increased the expression of heme oxygenase 1 (HO-1). Under ultraviolet B (UVB) and sodium nitroprusside (SNP) exposure, 3″Me-EGCG protected keratinocytes and regulated the survival protein Akt1. By regulating the Akt1/NF-κB pathway, 3″Me-EGCG augmented cell survival and proliferation in HaCaT cells. These results indicate that 3″Me-EGCG exhibits antioxidant properties, resulting in cytoprotection against various external stimuli. In conclusion, our findings suggest that 3″Me-EGCG can be used as an ingredient of cosmetic products or health supplements.

Keywords

(−)-Epigallocatechin-3-(3″-O-methyl) gallate (3″Me-EGCG); AKT1; antioxidant; cytoprotection.

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