(-)-Epigallocatechin-3-(3''-O-methyl) gallate (Synonyms: (-)-EGCG-3''-O-ME)

Name: (-)-Epigallocatechin-3-(3''-O-methyl) gallate
Brand: MedChemExpress
SKU: HY-N2228
Rating: 4.5 (1 reviews)

Cat. No.: HY-N2228 Purity: 99.83%: FAQs
Data Sheet SDS COA Handling Instructions

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(-)-Epigallocatechin-3-(3''-O-methyl) gallate ((-)-EGCG-3''-O-ME) is a natural product that can be isolated from the tea leaf, with strong antioxidative activity. (-)-Epigallocatechin-3-(3''-O-methyl) gallate has a strong cytotoxic activity for rat cancer cells.

For research use only. We do not sell to patients.

(-)-Epigallocatechin-3-(3''-O-methyl) gallate Chemical Structure

CAS No. : 83104-87-4

Size	Price	Stock	Quantity
1 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 480	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 670	In-stock	Estimated Time of Arrival: December 31
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100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Description

In Vitro

(-)-Epigallocatechin-3-(3''-O-methyl) gallate scavenges DPPH-derived radicals (IC₅₀: 36.54 μM), and scavenges ABTS radicals (IC₅₀: 2.59 μM)^[2].
(-)-Epigallocatechin-3-(3''-O-methyl) gallate (12.5 μM, 30 min) scavenges intracellular ROS production in RAW264.7 cells^[2].
(-)-Epigallocatechin-3-(3''-O-methyl) gallate (6.25 and 12 μM, 24-72 h) promotes cell proliferation in HaCaT cells^[2].
(-)-Epigallocatechin-3-(3''-O-methyl) gallate (6.25 and 12 μM, 12 h) increased HO-1 expression in HaCaT cells treated with 250 μM H₂O₂^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:	HaCaT cells treated with 250 μM H₂O₂
Concentration:	6.25 and 12 μM
Incubation Time:	24 h
Result:	Increased HO-1 expression, indicating the cytoprotective role against H₂O₂.

Molecular Weight

472.40

Formula

C₂₃H₂₀O₁₁

CAS No.

83104-87-4

Appearance

Solid

Color

Off-white to pink

SMILES

O=C(O[C@H]1[C@@H](C2=CC(O)=C(O)C(O)=C2)OC3=CC(O)=CC(O)=C3C1)C4=CC(OC)=C(O)C(O)=C4

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (105.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1169 mL	10.5843 mL	21.1685 mL
5 mM	0.4234 mL	2.1169 mL	4.2337 mL
10 mM	0.2117 mL	1.0584 mL	2.1169 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.65 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (2.65 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.83%

Select Batch:

Data Sheet (279 KB) SDS (252 KB)

COA (256 KB) HNMR (227 KB) CNMR (236 KB) RP-HPLC (230 KB) MS (117 KB)

Handling Instructions (2659 KB)

References

[1]. Kawase M, et al. Antioxidative activity of (-)-epigallocatechin-3-(3''-O-methyl)gallate isolated from fresh tea leaf and preliminary results on its biological activity. Biosci Biotechnol Biochem. 2000 Oct;64(10):2218-20. [Content Brief]

[2]. Kim E, et al. Antioxidant and Cytoprotective Effects of (-)-Epigallocatechin-3-(3″-O-methyl) Gallate. Int J Mol Sci. 2019 Aug 16;20(16):3993. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1169 mL	10.5843 mL	21.1685 mL	52.9213 mL
	5 mM	0.4234 mL	2.1169 mL	4.2337 mL	10.5843 mL
	10 mM	0.2117 mL	1.0584 mL	2.1169 mL	5.2921 mL
	15 mM	0.1411 mL	0.7056 mL	1.4112 mL	3.5281 mL
	20 mM	0.1058 mL	0.5292 mL	1.0584 mL	2.6461 mL
	25 mM	0.0847 mL	0.4234 mL	0.8467 mL	2.1169 mL
	30 mM	0.0706 mL	0.3528 mL	0.7056 mL	1.7640 mL
	40 mM	0.0529 mL	0.2646 mL	0.5292 mL	1.3230 mL
	50 mM	0.0423 mL	0.2117 mL	0.4234 mL	1.0584 mL
	60 mM	0.0353 mL	0.1764 mL	0.3528 mL	0.8820 mL
	80 mM	0.0265 mL	0.1323 mL	0.2646 mL	0.6615 mL
	100 mM	0.0212 mL	0.1058 mL	0.2117 mL	0.5292 mL

(-)-Epigallocatechin-3-(3''-O-methyl) gallate Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(-)-Epigallocatechin-3-(3''-O-methyl) gallate83104-87-4(-)-EGCG-3''-O-MEOthersantioxidativeanticancerHaCaTRAW264.7Inhibitorinhibitorinhibit

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(-)-Epigallocatechin-3-(3''-O-methyl) gallate (Synonyms: (-)-EGCG-3''-O-ME)

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Complete Stock Solution Preparation Table

(-)-Epigallocatechin-3-(3''-O-methyl) gallate Related Classifications

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