2. Academic Validation
  3. Molecular Mechanisms Underlying Yatein-Induced Cell-Cycle Arrest and Microtubule Destabilization in Human Lung Adenocarcinoma Cells

Molecular Mechanisms Underlying Yatein-Induced Cell-Cycle Arrest and Microtubule Destabilization in Human Lung Adenocarcinoma Cells

  • Cancers (Basel). 2019 Sep 17;11(9):1384. doi: 10.3390/cancers11091384.
Shang-Tse Ho 1 2,  Chi-Chen Lin 3,  Yu-Tang Tung 4 5 6,  Jyh-Horng Wu 7
Affiliations

Affiliations

  • 1 Department of Forestry, National Chung Hsing University, Taichung 402, Taiwan. s741129@hotmail.com.
  • 2 Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan. s741129@hotmail.com.
  • 3 Institute of Biomedical Science, National Chung Hsing University, Taichung 402, Taiwan. lincc@nchu.edu.tw.
  • 4 Graduate Institute of Metabolism and Obesity Sciences, Taipei Medical University, Taipei 110, Taiwan. f91625059@tmu.edu.tw.
  • 5 Nutrition Research Center, Taipei Medical University Hospital, Taipei 110, Taiwan. f91625059@tmu.edu.tw.
  • 6 Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, Taipei 116, Taiwan. f91625059@tmu.edu.tw.
  • 7 Department of Forestry, National Chung Hsing University, Taichung 402, Taiwan. eric@nchu.edu.tw.
PMID: 31533296 DOI: 10.3390/cancers11091384
Abstract

Yatein is an antitumor agent isolated from Calocedrus formosana Florin leaves extract. In our previous study, we found that yatein inhibited the growth of human lung adenocarcinoma A549 and CL1-5 cells by inducing intrinsic and extrinsic apoptotic pathways. To further uncover the effects and mechanisms of yatein-induced inhibition on A549 and CL1-5 cell growth, we evaluated yatein-mediated antitumor activity in vivo and the regulatory effects of yatein on cell-cycle progression and microtubule dynamics. Flow cytometry and western blotting revealed that yatein induces G2/M arrest in A549 and CL1-5 cells. Yatein also destabilized microtubules and interfered with microtubule dynamics in the two cell lines. Furthermore, we evaluated the antitumor activity of yatein in vivo using a xenograft mouse model and found that yatein treatment altered cyclin B/Cdc2 complex expression and significantly inhibited tumor growth. Taken together, our results suggested that yatein effectively inhibited the growth of A549 and CL1-5 cells possibly by disrupting cell-cycle progression and microtubule dynamics.

Keywords

Calocedrus formosana; cell-cycle arrest; lung cancer; xenograft; yatein.

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