2. Academic Validation
  3. Exendin‑4 promotes osteogenic differentiation of adipose‑derived stem cells and facilitates bone repair

Exendin‑4 promotes osteogenic differentiation of adipose‑derived stem cells and facilitates bone repair

  • Mol Med Rep. 2019 Dec;20(6):4933-4942. doi: 10.3892/mmr.2019.10764.
Banglian Deng 1,  Wenzhong Zhu 2,  Yansheng Duan 1,  Yuqian Hu 3,  Xuefeng Chen 4,  Shuang Song 5,  Zian Yi 6,  Yingliang Song 1
Affiliations

Affiliations

  • 1 State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi Key Laboratory of Stomatology, Department of Oral Implantation, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
  • 2 Department of Stomatology, Shaanxi Province Geriatric Hospital, Xi'an, Shaanxi 710005, P.R. China.
  • 3 Department of Stomatology, The Faculty of Medicine, Eastern University of Liaoning, Shenyang, Liaoning 110000, P.R. China.
  • 4 Xuefeng Dental Care Huaian, Huaian, Jiangsu 223000, P.R. China.
  • 5 Health Science Center, Peking University, Beijing 100000, P.R. China.
  • 6 Department of Stomatology, Kunming Medical University, Kunming, Yunnan 650500, P.R. China.
PMID: 31661134 DOI: 10.3892/mmr.2019.10764
Abstract

Inflammation‑related bone defects pose a heavy burden on patients and orthopedic surgeons. Although stem‑cell‑based bone repair has developed rapidly, it is of great significance to characterize bio‑active molecules that facilitate bone regeneration. It is reported that a glucagon‑like peptide 1 receptor agonist, exendin‑4, promoted bone regeneration mediated by the transplantation of adipose‑derived stem cells in a metaphyseal defect mouse model of femur injury. However, the underlying mechanism is unclear. Bone imaging, immunohistochemistry real‑time PCR and western blot analysis were used in the present study, and the results revealed that exendin‑4 increased the transcription of the osteogenic differentiation‑related genes and induced osteogenic differentiation in situ. Furthermore, the present data obtained from sorted adipose‑derived stem cells revealed that exendin‑4 promoted osteogenic differentiation and inhibited adipogenic differentiation in vitro. These findings indicated that exendin‑4 facilitates osteogenic differentiation of transplanted adipose‑derived stem cells for bone repair and illuminated clinical prospects of both adipose‑derived stem cells and exendin‑4 in stem‑cell‑based bone defect repair.

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