2. Academic Validation
  3. Separation, purification, structural analysis and immune-enhancing activity of sulfated polysaccharide isolated from sea cucumber viscera

Separation, purification, structural analysis and immune-enhancing activity of sulfated polysaccharide isolated from sea cucumber viscera

  • Int J Biol Macromol. 2020 Jul 15;155:1003-1018. doi: 10.1016/j.ijbiomac.2019.11.064.
Dongda Yang 1,  Fudi Lin 1,  Yayan Huang 1,  Jing Ye 2,  Meitian Xiao 3
Affiliations

Affiliations

  • 1 College of Chemical Engineering, Huaqiao university of China, Xiamen 361021, China.
  • 2 College of Chemical Engineering, Huaqiao university of China, Xiamen 361021, China; Xiamen Engineering and Technological Research Center for Comprehensive Utilization of Marine Biological Resources, Xiamen 361021, Fujian, China. Electronic address: yejenny@hqu.edu.cn.
  • 3 College of Chemical Engineering, Huaqiao university of China, Xiamen 361021, China; Xiamen Engineering and Technological Research Center for Comprehensive Utilization of Marine Biological Resources, Xiamen 361021, Fujian, China. Electronic address: mtxiao@hqu.edu.cn.
PMID: 31712137 DOI: 10.1016/j.ijbiomac.2019.11.064
Abstract

A novel sulfated polysaccharide (SCVP-1) was isolated from sea cucumber viscera and purified to elucidate its structure and immune-enhancing ability. SCVP-1 was found to be a homogeneous polysaccharide with a relative molecular weight of 180.8 kDa and composed of total sugars (60.2 ± 2.6%), uronic acid (15.3 ± 1.8%), proteins (6.8 ± 0.8%), and sulfate groups (18.1 ± 0.9%). SCVP-1 consisted of mannose, glucosamine, glucuronic acid, N-acetyl-galactosamine, glucose, galactose and fucose at an approximate molar ratio of 1.00:1.41:0.88:2.14:1.90:1.12:1.24. The fourier transform infrared spectra (FT-IR) and nuclear magnetic resonance (NMR) analyses showed that SCVP-1 was a kind of glycosaminoglycan. And the sulfation patterns of the fucose branches were Fuc2,4S, Fuc3,4S and Fuc0S. The surface morphology of SCVP-1 presented loose and irregular sheet structure formed by aggregation of polysaccharide molecules with spherical structure. Moreover, SCVP-1 promoted the production of nitric oxide (NO) and cytokines (IL-1β, IL-6 and TNF-α) by RAW264.7 cells as well as the expression of related genes (iNOS, IL-1β, IL-6 and TNF-α) and also enhanced their phagocytic activity through TLR4-mediated activation of the MAPKs and NF-κB signaling pathways. This study suggests that sea cucumber viscera are good sources of Polysaccharides and SCVP-1 might be a novel immunomodulator.

Keywords

Immune enhancement; RAW264.7 macrophages; Sea cucumber viscera; Structure; Sulfated polysaccharide.

Figures
Products