2. Academic Validation
  3. Triterpenoids with anti-proliferative effects from the seeds of Peganum harmala L

Triterpenoids with anti-proliferative effects from the seeds of Peganum harmala L

  • Phytochemistry. 2020 Jun;174:112342. doi: 10.1016/j.phytochem.2020.112342.
Haiyan Li 1,  Zhe Wang 1,  Yihai Wang 2,  Jingwen Xu 3,  Xiangjiu He 4
Affiliations

Affiliations

  • 1 School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China.
  • 2 School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China; Guangdong Engineering Research Center for Lead Compounds & Drug Discovery, Guangzhou, 510006, China. Electronic address: wangyih88@163.com.
  • 3 School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China; Guangdong Engineering Research Center for Lead Compounds & Drug Discovery, Guangzhou, 510006, China.
  • 4 School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China; Guangdong Engineering Research Center for Lead Compounds & Drug Discovery, Guangzhou, 510006, China. Electronic address: hexiangjiu@163.com.
PMID: 32172018 DOI: 10.1016/j.phytochem.2020.112342
Abstract

Three undescribed lupane-type triterpenoids (1-3), three undescribed oleanane-type triterpenoids (4-6), and ten known Pentacyclic Triterpenoids (7-16) were isolated from the seeds of Peganum harmala L. (Zygophyllaceae). Their structures were elucidated using various spectroscopic methods (IR, HR-ESI-MS, 1D, and 2D NMR). All the triterpenoids were screened for anti-proliferative activity against HeLa, HepG2, and SGC-7901 cells using the MTT method. Except compounds 1, 2, and 13, all the Other triterpenoids exhibited potent cytotoxic activities against tumour cells. Compounds 3, 6, and 15 inhibited the proliferation of HeLa cells in a dose-dependent manner, as observed by the colony formation assay. When HeLa cells were treated with different doses of compounds 3, 6, and 15, the cell nuclei changed shape to a crescent form and were condensed and fragmented, as observed by Hoechst 33258 staining. Additionally, these three triterpenoids induced the Apoptosis in HeLa cells, which was detected by Western blot analysis.

Keywords

Anti-proliferative; Apoptosis; Peganum harmala L.; Triterpenoids; Zygophyllaceae.

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