2. Academic Validation
  3. A sequential methodology for integral evaluation of motor and non-motor behaviors in parkinsonian rodents

A sequential methodology for integral evaluation of motor and non-motor behaviors in parkinsonian rodents

  • MethodsX. 2020 Feb 20;7:100821. doi: 10.1016/j.mex.2020.100821.
Luis O Soto-Rojas 1 2,  Cecilia Bañuelos 3,  Linda Garces-Ramirez 1,  Claudia Luna-Herrera 1,  Yazmin M Flores-Martínez 4,  Guadalupe Soto-Rodríguez 5,  Bismark Gatica-García 4,  Francisco E López-Salas 6,  José Ayala-Dávila 4,  María E Gutiérrez-Castillo 7,  América Padilla-Viveros 3,  Fidel de la Cruz-López 1,  Irma A Martínez-Davila 4,  Daniel Martinez-Fong 4 6
Affiliations

Affiliations

  • 1 Departamento de Fisiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu s/n, Unidad Profesional "Adolfo López Mateos", Ciudad de México 07738, México.
  • 2 Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios No. 1, Los Reyes Iztacala, Tlalnepantla, Edo. de México 54090, México.
  • 3 Coordinación General de Programas Multidisciplinarios. Programa Transdisciplinario en Desarrollo Científico y Tecnológico para la Sociedad, Av. Instituto Politécnico Nacional No. 2508, Centro de Investigación y de Estudios Avanzados, Ciudad de México 07360, México.
  • 4 Departamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados, Av. Instituto Politécnico Nacional No. 2508, Ciudad de México 07360, México.
  • 5 Facultad de Medicina, Benemérita Universidad Autónoma de Puebla, 13 Sur 2702, Puebla 72420, México.
  • 6 Programa de Doctorado en Nanociencias y Nanotecnología, Av. Instituto Politécnico Nacional No. 2508, Centro de Investigación y de Estudios Avanzados, Ciudad de México 07360, México.
  • 7 Departamento de Biociencias e Ingeniería, Centro Interdisciplinario de Investigaciones y Estudios sobre Medio Ambiente y Desarrollo, Instituto Politécnico Nacional, 30 de junio de 1520s/n, Ciudad de México 07340, México.
PMID: 32195138 DOI: 10.1016/j.mex.2020.100821
Abstract

An animal model, suitable for resembling Parkinson's disease (PD) progress, should show both, motor and non-motor alterations. However, these features have been scarcely evaluated or developed in parkinsonian models induced by neurotoxins. This protocol provides modifications to original methods, allowing six different motor and non-motor behavior tests, which adequately and timely emulate the main parkinsonian sensorimotor alterations in the rat or mouse: (1) bilateral sensorimotor alterations, examined by the vibrissae test; (2) balance and motor coordination, evaluated by the uncoordinated gait test; (3) locomotor asymmetry, analyzed by the cylinder test; (4) bradykinesia, as a locomotor alteration evidenced by the open field test; (5) depressive-like behavior, judged by the forced swimming test; and (6) hyposmia, assessed by the olfactory asymmetry test. Some advantages of using these behavioral tests over Others include:•No sophisticated Materials or equipment are required for their application and evaluation.•They are used in rodent models for parkinsonian research, but they can also be helpful for studying Other movement disorders.•These tests can accurately discriminate the affected side from the healthy one, after unilateral injury of one hemisphere, resulting in sensorimotor, olfactory or locomotor asymmetry.

Keywords

6-OHDA; BSSG; Bradykinesia; Depressive like-behavior; Locomotor asymmetry; Parkinsonism; Sensorimotor alteration; Uncoordinated gait.

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