2. Academic Validation
  3. Resveratrol prevents benzo(a)pyrene-induced disruption of mitochondrial homeostasis via the AMPK signaling pathway in primary cultured neurons

Resveratrol prevents benzo(a)pyrene-induced disruption of mitochondrial homeostasis via the AMPK signaling pathway in primary cultured neurons

  • Environ Pollut. 2020 Jun;261:114207. doi: 10.1016/j.envpol.2020.114207.
Run-Run Kang 1,  Qian Sun 2,  Kai-Ge Chen 3,  Qing-Tian Cao 4,  Chang Liu 5,  Kuan Liu 6,  Zhuo Ma 7,  Yu Deng 8,  Wei Liu 9,  Bin Xu 10
Affiliations

Affiliations

  • 1 Department of Environmental Health, School of Public Health, China Medical University, Shenyang, 110122, Liaoning, China. Electronic address: kangrunrun0310@163.com.
  • 2 Department of Environmental Health, School of Public Health, China Medical University, Shenyang, 110122, Liaoning, China. Electronic address: sq17cmu@163.com.
  • 3 Department of Environmental Health, School of Public Health, China Medical University, Shenyang, 110122, Liaoning, China. Electronic address: 18040016591@163.com.
  • 4 Department of Environmental Health, School of Public Health, China Medical University, Shenyang, 110122, Liaoning, China. Electronic address: caoqingtian@163.com.
  • 5 Department of Environmental Health, School of Public Health, China Medical University, Shenyang, 110122, Liaoning, China. Electronic address: lc_cmu111@163.com.
  • 6 Department of Environmental Health, School of Public Health, China Medical University, Shenyang, 110122, Liaoning, China. Electronic address: lk_cmu@163.com.
  • 7 Department of Environmental Health, School of Public Health, China Medical University, Shenyang, 110122, Liaoning, China. Electronic address: mz_cmu@163.com.
  • 8 Department of Environmental Health, School of Public Health, China Medical University, Shenyang, 110122, Liaoning, China. Electronic address: dengyu.cmu@163.com.
  • 9 Department of Environmental Health, School of Public Health, China Medical University, Shenyang, 110122, Liaoning, China. Electronic address: liuw@cmu.edu.cn.
  • 10 Department of Environmental Health, School of Public Health, China Medical University, Shenyang, 110122, Liaoning, China. Electronic address: bxu10@cmu.edu.cn.
PMID: 32220751 DOI: 10.1016/j.envpol.2020.114207
Abstract

Exposure to benzo(a)pyrene (BaP) has been shown to cause mitochondrial dysfunction and injury to neural cells. Resveratrol (RSV) has been studied as an antioxidant, anti-inflammatory, anti-apoptotic, and Anticancer agent and can modulate mitochondrial function in vitro and in vivo. However, the molecular mechanisms underlying RSV's protection against mitochondrial dysfunction have not been fully elucidated. To investigate whether RSV can effectively prevent BaP-induced mitochondrial dysfunction, we tested the effects of RSV in primary neuronal models. Our results confirmed that neurons exhibited mitochondrial dysfunction and Apoptosis in the mitochondrial pathway after BaP-treatment, and that pretreatment with RSV could reduce that dysfunction. Further, our results indicated that RSV pretreatment enhanced mitochondrial biogenesis via the AMPK/PGC-1α pathway and activated Mitophagy via the PINK1-Parkin and AMPK/ULK1 pathways, thereby coordinating mitochondrial homeostasis. We also found that RSV could alleviate mitochondrial network fragmentation caused by BaP. This work provided insights into the role of RSV in preventing BaP-induced primary neuronal Apoptosis in the mitochondrial pathway, mainly via regulation of mitochondrial biogenesis and Mitophagy through AMPK pathway, thus maintaining the integrity of the mitochondrial network.

Keywords

Adenosine monophosphate-activated protein kinase; Benzo(a)pyrene; Mitochondrial homeostasis; Neurotoxicity; Resveratrol.

Figures
Products